Overview

  • Product name
    Anti-CNBP antibody
    See all CNBP primary antibodies
  • Description
    Rabbit polyclonal to CNBP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (SSNECFKCGR SGHWARECPT GGGRGRGMRS RGRGGFTSDR GFQFVSSSLP) of Human CNBP (NP_003409).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human fetal brain lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1.777777778.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 19 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Single stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression.
  • Tissue specificity
    Present in all tissues examined.
  • Involvement in disease
    Defects in CNBP are the cause of dystrophia myotonica type 2 (DM2) [MIM:602668]; also known as proximal myotonic myopathy (PROMM). A multisystem disease characterized by the association of proximal muscle weakness with myotonia, cardiac manifestations and cataract. Additional features can include hyperhidrosis, testicular atrophy, insulin resistance and diabetes and central nervous system anomalies in rare cases. Note=The causative mutation is a CCTG expansion (mean approximately 5000 repeats) located in intron 1 of the CNBP gene.
  • Sequence similarities
    Contains 7 CCHC-type zinc fingers.
  • Cellular localization
    Cytoplasm. Endoplasmic reticulum.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • CCHC type zinc finger nucleic acid binding protein antibody
    • Cellular nucleic acid binding protein antibody
    • Cellular nucleic acid-binding protein antibody
    • CNBP antibody
    • CNBP_HUMAN antibody
    • CNBP1 antibody
    • DM2 antibody
    • Erythroid differentiation related antibody
    • PROMM antibody
    • Proximal myotonic myopathy nucleic acid binding protein antibody
    • RNF163 antibody
    • Sterol regulatory element binding protein antibody
    • ZCCHC22 antibody
    • Zinc finger protein 273 antibody
    • Zinc finger protein 9 (a cellular retroviral nucleic acid binding protein) antibody
    • Zinc finger protein 9 antibody
    • ZNF9 antibody
    see all

Images

  • Western blot - CNBP antibody (ab83038)
    Western blot - CNBP antibody (ab83038)
    Anti-CNBP antibody (ab83038) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 19 kDa
    Observed band size: 21 kDa (why is the actual band size different from the predicted?)



    Gel concentration: 12%

References

This product has been referenced in:
  • Lee E  et al. CNBP acts as a key transcriptional regulator of sustained expression of interleukin-6. Nucleic Acids Res 45:3280-3296 (2017). Read more (PubMed: 28168305) »

See 1 Publication for this product

Publishing research using ab83038? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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3 reviews or Q&A

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Western blot abreview for Anti-CNBP antibody

 Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (bone marrow derived macrophages)
Gel Running Conditions
Reduced Denaturing (13%)
Loading amount
500000 cells
Specification
bone marrow derived macrophages
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
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Abcam user community

Verified customer

Submitted Jan 18 2016

Thank you for your reply to my inquiry. We would like to try ab83038 antibodies for immunofluorescence (IF) under your offer conditions in my lab. As soon as we receive the appropriate discount code from you, we will order the antibody (hopefully later...

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I am very pleased to hear you would like to accept our offer and test ab83038 in ICC/IF. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ICC/IF and include this code in...

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ab83038 and ab48027: Both of these antibodies are aganist CNBP. Your data sheet o each sayu that each antibody has been tried and works in WB abd ELISA. Other applications have not been tried. However, your spec sheets say both give antigen localizatio...

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Thank you very much for your interest in our anti-CNBP antibodies. To our knowledge, neither ab48027 nor ab83038 have been tested in cell staining (immunocytochemistry, ICC/IF). The information about localization in the ER and cytoplasm comes f...

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