- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-CNBP antibody
See all CNBP primary antibodies -
DescriptionRabbit polyclonal to CNBP
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Host speciesRabbit
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Tested applicationsSuitable for: ELISA, WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Cat, Dog
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Immunogen
Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (SSNECFKCGR SGHWARECPT GGGRGRGMRS RGRGGFTSDR GFQFVSSSLP) of Human CNBP (NP_003409).
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Positive control
- Human fetal brain lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab83038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. Titre using peptide based assay: 1.777777778. |
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| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 19 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
Target
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FunctionSingle stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). Involved in sterol-mediated repression.
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Tissue specificityPresent in all tissues examined.
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Involvement in diseaseDefects in CNBP are the cause of dystrophia myotonica type 2 (DM2) [MIM:602668]; also known as proximal myotonic myopathy (PROMM). A multisystem disease characterized by the association of proximal muscle weakness with myotonia, cardiac manifestations and cataract. Additional features can include hyperhidrosis, testicular atrophy, insulin resistance and diabetes and central nervous system anomalies in rare cases. Note=The causative mutation is a CCTG expansion (mean approximately 5000 repeats) located in intron 1 of the CNBP gene.
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Sequence similaritiesContains 7 CCHC-type zinc fingers.
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Cellular localizationCytoplasm. Endoplasmic reticulum.
- Information by UniProt
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Database links
- Entrez Gene: 7555 Human
- Entrez Gene: 12785 Mouse
- Entrez Gene: 64530 Rat
- Omim: 116955 Human
- SwissProt: P62633 Human
- SwissProt: P53996 Mouse
- SwissProt: P62634 Rat
- Unigene: 518249 Human
see all -
Alternative names
- CCHC type zinc finger nucleic acid binding protein antibody
- Cellular nucleic acid binding protein antibody
- Cellular nucleic acid-binding protein antibody
see all
Images
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Western blot - Anti-CNBP antibody (ab83038)Anti-CNBP antibody (ab83038) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 19 kDa
Observed band size: 21 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
Protocols
References
This product has been referenced in:
- Lee E et al. CNBP acts as a key transcriptional regulator of sustained expression of interleukin-6. Nucleic Acids Res 45:3280-3296 (2017). Read more (PubMed: 28168305) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"