Anti-CNKSR3 antibody (ab72978)

Overview

  • Product nameAnti-CNKSR3 antibody
    See all CNKSR3 primary antibodies
  • Description
    Mouse polyclonal to CNKSR3
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-555 of Human CNKSR3, (NP_775786).

  • Positive control
    • CNKSR3 transfected 293T cell lysate. HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesICC/IF: Use at a concentration of 10 µg/ml.
    Protein A purify ab72978 before commencing IF/ICC procedure.
    WB: 1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceCNKSR3 belongs to the CNKSR family. It contains 1 CRIC domain, 1 DUF1170 domain, 1 PDZ (DHR) domain and 1 SAM (sterile alpha motif) domain.
    • Cellular localizationCell Membrane and Cytoplasmic
    • Database links
    • Alternative names
      • CNK homolog protein 3 antibody
      • CNK3 antibody
      • CNKSR family member 3 antibody
      • Connector enhancer of kinase suppressor of ras 3 antibody
      • Connector enhancer of KSR 3 antibody
      • MAGI1 antibody
      • Maguin like protein antibody
      • Membrane associated guanylate kinase interacting protein like 1 antibody
      • Membrane associated guanylate kinase, WW and PDZ domain containing 1 antibody
      • RP11 486M3 1 antibody
      • RP11 486M3.1 antibody
      see all

    Anti-CNKSR3 antibody images

    • HeLa cells staining CNKSR3, using Protein A purified ab72978 at 10µg/ml.
    • All lanes : Anti-CNKSR3 antibody (ab72978) at 1/500 dilution

      Lane 1 : CNKSR3 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size : 62 kDa
      Observed band size : 62 kDa

    References for Anti-CNKSR3 antibody (ab72978)

    ab72978 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your reply and all the additional information. This makes the problems you have been experiencing much clearer to understand. It is clear that you have spent a lot of time in trying to optimise these antibodies and I am sorry that they ar...

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    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Colon)
    Specification Colon
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Flex High pH
    Permeabilization No
    Blocking step Dako Flex as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 06 2012

    I am very pleased to hear you would like to accept our offer and test ab72978 in IHC-P.

    DISCOUNT CODE: ####
    Expiration date: ####

    This code will give you 1 free primary antibody before the expiration date. To redeem this offe...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"