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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Purification notes The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
1/500 - 1/1000. Predicted molecular weight: 34 kDa.
Function Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Tissue specificity Heart and smooth muscle.
Sequence similarities Belongs to the calponin family. Contains 3 calponin-like repeats. Contains 1 CH (calponin-homology) domain.
Information by UniProt
Calponin 2 antibody
Calponin H2 antibody
Calponin H2, smooth muscle antibody
Anti-CNN2 antibody images
Western blot - CNN2 antibody (ab75003)
All lanes : Anti-CNN2 antibody (ab75003) at 1/500 dilution Lane 1 : HUVEC extract Lane 2 : HUVEC extract with immunising peptide at 10 µg Lysates/proteins at 5 µg per lane. Predicted band size : 34 kDa Observed band size : 34 kDa Additional bands at : 28 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - CNN2 antibody (ab75003)
Immunofluorescence analysis of CNN2 in A549 cells, using ab75003. Right panel was also treated with immunising peptide.
References for Anti-CNN2 antibody (ab75003)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"