Overview

  • Product name
    Anti-CNNM1 antibody
  • Description
    Rabbit polyclonal to CNNM1
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence:TACHMDSSPQSPDMEAFTDGDSTKAPTTRGTPQTPKDDPAI TLLNNRNSLPCSRSDGLRS PSEVVYLRMEELAFTQEEMTDFEEHSTQQLT, corresponding to amino acids 791-881 of Human CNNM1 Isoform 1 (Q9NRU3).

  • Positive control
    • Human testis tissue Human plasma tissue Human liver tissue lysate Human tonsil tissue lysate RT 4 cell line lysate U 251 MG cell line lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab122648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Probable metal transporter.
  • Tissue specificity
    Restricted to brain and testis.
  • Sequence similarities
    Belongs to the ACDP family.
    Contains 2 CBS domains.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACDP1 antibody
    • Ancient conserved domain protein 1 antibody
    • Ancient conserved domain-containing protein 1 antibody
    • CNNM1 antibody
    • CNNM1_HUMAN antibody
    • Cyclin-M1 antibody
    • FLJ31632 antibody
    • Metal transporter CNNM1 antibody
    • OTTHUMP00000020253 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab122648 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-CNNM1 antibody (ab122648) at 1/250 dilution

    Lane 1 : RT 4 cell line lysate
    Lane 2 : U 251 MG cell line lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate
  • ab122648, at 1/1000, staining CNNM1 in paraffin embedded Human testis by Immunohistochemistry.

References

ab122648 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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