Anti-CNOT2 antibody (ab55679)

Overview

  • Product nameAnti-CNOT2 antibody
    See all CNOT2 primary antibodies
  • Description
    Mouse monoclonal to CNOT2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment: EDLLFYLYYM NGGDVLQLLA AVELFNRDWR YHKEERVWIT RAPGMEPTMK TNTYERGTYY FFDCLNWRKV AKEFHLEYDK LEERPHLPST FNYNPAQQAF , corresponding to amino acids 441-541 of Human CNOT2

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab55679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 60 kDa.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionThe CCR4-NOT complex functions as general transcription regulation complex.
  • Tissue specificityUbiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the CNOT2/3/5 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCR4 associated factor 2 antibody
    • CCR4 NOT transcription complex subunit 2 antibody
    • CCR4-associated factor 2 antibody
    • CCR4-NOT transcription complex subunit 2 antibody
    • CDC36 antibody
    • CNOT2 antibody
    • CNOT2_HUMAN antibody
    • HSPC131 antibody
    • MSTP046 antibody
    • Negative regulator of transcription 2 antibody
    • NOT2 (negative regulator of transcription 2 yeast) homolog antibody
    • NOT2 antibody
    • NOT2H antibody
    see all

Anti-CNOT2 antibody images



  • Predicted band size : 60 kDa
    CNOT2 antibody (ab55679) at 1ug/lane + A-549 cell lysate at 25ug/lane.
  • ab55679 staining CNOT2 in mouse ES cells by Immunocytochemistry/ Immunofluorescence. The cells were methanol fixed and then blocked using 0.2% fish scale gelatin for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/300 for 20 minutes at 25°C. The secondary antibody used was a donkey anti-mouse IgG conjugated to Alexa Fluor® 594 (red) used at a 1/500 dilution. DAPI was used to stain the cell nuclei (blue).

    See Abreview

References for Anti-CNOT2 antibody (ab55679)

ab55679 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 30 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (11)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 16 2012

Vielen Dank für Ihre Email. Es tut mir leid zu hören, dass Sie Probleme mit diesem Antikörper haben. Wir haben zur Zeit kein anders Feedback bekommen, dass es ein Problem hätte mit diesem Antikörper. Wir nehmen jedoch alle Produktbeschwerden se...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mouse ES cells)
Specification mouse ES cells
Fixative Methanol
Permeabilization No
Blocking step Fish scale gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"