Overview

  • Product name
  • Description
    Rabbit polyclonal to CNOT3
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit
  • Immunogen

    Recombinant fragment corresponding to Human CNOT3 aa 522-753 (C terminal).
    Database link: O75175

  • Positive control
    • IMR32 whole cell lysate; NT2D1, Jurkat and HeLa cell lines; Human H441 xenograft tissue; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab154276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 82 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    The CCR4-NOT complex functions as general transcription regulation complex.
  • Tissue specificity
    Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the CNOT2/3/5 family.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCR4 associated factor 3 antibody
    • CCR4-associated factor 3 antibody
    • CCR4-NOT transcription complex subunit 3 antibody
    • CNOT3 antibody
    • CNOT3_HUMAN antibody
    • KIAA0691 antibody
    • LENG2 antibody
    • Leukocyte receptor cluster member 2 antibody
    • negative regulator of transcription 3, S. cerevisiae, homolog of antibody
    • NOT3 (negative regulator of transcription 3, yeast) homolog antibody
    • NOT3 antibody
    • NOT3H antibody
    see all

Images

  • Anti-CNOT3 antibody (ab154276) at 1/3000 dilution + IMR32 whole cell lysate at 30 µg

    Predicted band size : 82 kDa
    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labeling CCR4 NOT transcription complex subunit 3 with ab154276 at 1/200 dilution. Lower panel: Costained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human H441 xenograft, labeling CCR4 NOT transcription complex subunit 3 with ab154276 at 1/100 dilution.

References

ab154276 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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