Overview

  • Product nameAnti-CNOT4 antibody
    See all CNOT4 primary antibodies
  • Description
    Rabbit polyclonal to CNOT4
  • SpecificityDetects endogenous levels of total CNOT4 protein
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthesized peptide derived from an internal sequence of human CNOT4.

  • Positive control
    • Extracts from Jurkat or K562 cells; human testis tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab110013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 63 kDa.
IHC-P 1/50 - 1/100.

Target

  • FunctionHas E3 ubiquitin ligase activity. The CCR4-NOT complex functions as general transcription regulation complex.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 C3H1-type zinc finger.
    Contains 1 RING-type zinc finger.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Autoubiquitinated.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCR4 NOT transcription complex subunit 4 antibody
    • CCR4-associated factor 4 antibody
    • CCR4-NOT transcription complex subunit 4 antibody
    • CLONE243 antibody
    • CNOT 4 antibody
    • Cnot4 antibody
    • CNOT4_HUMAN antibody
    • E3 ubiquitin-protein ligase CNOT4 antibody
    • NOT 4 antibody
    • NOT4 (negative regulator of transcription 4 yeast) homolog antibody
    • NOT4 antibody
    • NOT4H antibody
    • Potential transcriptional repressor NOT4Hp antibody
    see all

Anti-CNOT4 antibody images

  • Immunohistochemistry analysis of paraffin embedded human testis tissue, using ab110013 at 1/50. The picture on the right is treated with the synthesized peptide.
  • All lanes : Anti-CNOT4 antibody (ab110013) at 1/500 dilution

    Lane 1 : extracts from K562 cells (5-30 ug) with no immunizing peptide
    Lane 2 : extracts from Jurkat cells (5-30 ug) with no immunizing peptide
    Lane 3 : extracts from K562 cells (5-30 ug) with immunizing peptide (5-10 ug)


    Predicted band size : 63 kDa

References for Anti-CNOT4 antibody (ab110013)

ab110013 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"