Overview

  • Product name
    Anti-CNOT7 antibody [EPR18722]
    See all CNOT7 primary antibodies
  • Description
    Rabbit monoclonal [EPR18722] to CNOT7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Mouse CNOT7 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q60809

  • Positive control
    • WB: Mouse ovary, embryo, brain and heart lysates; F9, SP2/0, C6, PC-12, HeLa, HepG2, NTERA-2/D1 and NCCIT whole cell lysates; rat kidney, spleen and heart lysates; human fetal brain, fetal heart, fetal kidney and fetal spleen lysates. IP: HeLa whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR18722
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab195587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.
Flow Cyt 1/1000.
IP 1/40.
WB 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).

Target

  • Function
    Ubiquitous transcription factor required for a diverse set of processes. It is a component of the CCR4 complex involved in the control of gene expression.
  • Sequence similarities
    Belongs to the CAF1 family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BTG1 binding factor 1 antibody
    • BTG1-binding factor 1 antibody
    • CAF 1 antibody
    • CAF-1 antibody
    • CAF1 antibody
    • Carbon catabolite repressor protein (CCR4) associative factor 1 antibody
    • CCR4 associated factor 1 antibody
    • CCR4 NOT transcription complex subunit 7 antibody
    • CCR4-associated factor 1 antibody
    • CCR4-NOT transcription complex subunit 7 antibody
    • CNOT 7 antibody
    • Cnot7 antibody
    • CNOT7_HUMAN antibody
    • hCAF 1 antibody
    • hCAF1 antibody
    see all

Images

  • All lanes : Anti-CNOT7 antibody [EPR18722] (ab195587) at 1/1000 dilution

    Lane 1 : Mouse ovary lysate
    Lane 2 : Mouse embryo lysate
    Lane 3 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate
    Lane 4 : SP2/0 (Mouse spleen cell line) whole cell lysate
    Lane 5 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa
    Observed band size: 33 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 2 seconds; Lane 2-6: 5 seconds.

  • All lanes : Anti-CNOT7 antibody [EPR18722] (ab195587) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Rat kidney lysate
    Lane 4 : Rat spleen lysate
    Lane 5 : Rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa
    Observed band size: 33 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/2: 15 seconds; Lane 3/4: 5 seconds;  Lane 5: 30 seconds.

     

  • All lanes : Anti-CNOT7 antibody [EPR18722] (ab195587) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate
    Lane 4 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 33 kDa
    Observed band size: 33 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • All lanes : Anti-CNOT7 antibody [EPR18722] (ab195587) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 3 : NTERA-2/D1 (Human malignant pluripotent embryonic carcinoma cell line) whole cell lysate
    Lane 4 : NCCIT (Human pluripotent embryonic carcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa
    Observed band size: 33 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 seconds; Lane 2-4: 2 seconds.

     

  • CNOT7 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab195587 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab195587 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate 10µg (Input).

    Lane 2: ab195587 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab195587 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling CNOT7 with ab195587 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab195587at 1/1000 dilution, followed by Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary at 1/XXXX dilution.
    -ve control 2:  Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary at 1/1000 dilution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized F9 (Mouse embryonic testicular cancer cell line) cells labeling CNOT7 with ab195587 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab195587at 1/1000 dilution, followed by Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary at 1/XXXX dilution.
    -ve control 2:  Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary at 1/1000 dilution.

  • Flow cytometry analysis of 4% paraformaldehyde fixed NIH/3T3 (Mouse embryonic fibroblast cell line) cell line labelling CNOT7 with ab195587 at 1/1000 dilution (red), isotype control with ab172730 (black) and unlabelled control cells without incubation with primary antibody and secondary antibody (blue).

  • Flow cytometry analysis of 4% paraformaldehyde fixed F9 (Mouse embryonic testicular cancer cell line) cells labeling CNOT7 with ab195587 at 1/1000 dilution (red), isotype control with ab172730 (black) and unlabeled control cells without incubation with primary antibody and secondary antibody (blue).

References

This product has been referenced in:
  • Yu C  et al. BTG4 is a meiotic cell cycle-coupled maternal-zygotic-transition licensing factor in oocytes. Nat Struct Mol Biol N/A:N/A (2016). Read more (PubMed: 27065194) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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