Overview

  • Product name
  • Description
    Chicken polyclonal to CNPase
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Peptide common to human (NP_149124, NCBI) and mouse (P16330, NCBI) CNPase.

  • Positive control
    • Adult mouse brain

Properties

Applications

Our Abpromise guarantee covers the use of ab50739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
IHC-P Use at an assay dependent concentration.

Target

Images

  • Immunohistochemistry of a section through an adult mouse brain showing CNPase (brown staining) in white matter tracts and the granule cell layer of the cerebellum using ab50739.

References

This product has been referenced in:
  • Wheeler NA  et al. HIV-1 Tat Inhibits Autotaxin Lysophospholipase D Activity and Modulates Oligodendrocyte Differentiation. ASN Neuro 8:N/A (2016). Mouse . Read more (PubMed: 27659560) »
  • Costin BN  et al. Ethanol regulation of serum glucocorticoid kinase 1 expression in DBA2/J mouse prefrontal cortex. PLoS One 8:e72979 (2013). IHC . Read more (PubMed: 23991167) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 37°C
Sample
Human Tissue sections (Spinal Cord)
Specification
Spinal Cord
Permeabilization
Yes - 0.5% Triton X-100
Fixative
Paraformaldehyde
Username

Mr. Chris Hewitt

Verified customer

Submitted Mar 03 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% tween
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 07 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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