CNPase antibody [SMI-91] (ab24566)

Product Name 

CNPase antibody [SMI-91]

Product type 

Primary antibodies

Description 

Mouse monoclonal [SMI-91] to CNPase

Immunogen 

Purified Human Brain CNPase.

Reacts with 

Hu, Ms, Rat, Cow, Dog, Pig, Shp

Does not react with 
(species key)

Bird, Gpig

Specificity 

ab24566 reacts with the 46 and 48 Mr subunit of the 94 Mr myelin CNPase dimer. It detects developing and adult myelin, developing oligodendrocytes and Schwann cells and distinguishes oligo endrocytes from astrocytes, microglia, neurons and other cells in brain sections.

Tested applications 
(see key)

ELISA, IHC-FoFr, IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: 1/200.
IHC-P: 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: 1/200 (PMID 19799876).
IHC-FoFr: 1/200.
WB: 1/200. Predicted molecular weight: 47 kDa.

Note: Post-fixation in cold methanol, methanol/hydrogen peroxide or detergent is necessary for access of ab24566 to oligodendrocytes and myelin in frozen and thick Vibratome tissue sections or in tissue culture. Reaction on formalin-fixed, paraffin-embedded sections may be enhanced by autoclaving deparaffinized sections in distilled water for 10 min or by boiling sections or tissue blocks immersed in tris buffered saline, pH 9.0, in a microwave oven for 15 min.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell Membrane: bound to membrane structures of brain white matter.

Research areas 

Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker

Relevance 

CNPase (2',3'-Cyclic Nucleotide 3'-Phosphodiesterase) exists in two forms: 46kDa (CNP1) and 48kDa (CNP2). It is expressed at high levels by oligodendrocytes in the central nervous system and by Schwann cells in the peripheral nervous system and is virtual absent in other cell types. CNPase activity is decreased in demyelinating diseases such as multiple sclerosis. CNPase gene is localized to chromosome 17q21, very near the BRCA 1 familial breast cancer gene.

Ab 24566 is useful for immunocytochemical studies on the progression of normal and pathologic myelination.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

SMI-91

Isotype 

IgG1

Purity 

Ascites

Storage buffer 

Preservative: 0.01M Sodium Azide
Constituents: Ascites

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

See below for CNPase antibody [SMI-91] images, references, products related to ab24566 and other tools.

CNPase antibody [SMI-91] images:

References for CNPase antibody [SMI-91] (ab24566)

  This product has been used in: (two most recent references)

Huang Z et al. Long-term cognitive impairment and myelination deficiency in a rat model of perinatal hypoxic-ischemic brain injury. Brain Res 1301:100-9 (2009). IHC-FoFr; Rat. PubMed: 19747899

Pott F et al. Cuprizone effect on myelination, astrogliosis and microglia attraction in the mouse basal ganglia. Brain Res 1305:137-49 (2009). IHC-FoFr; Mouse. PubMed: 19799876

See all 3 publication references for this product.


If you publish research using ab24566 please let us know so that we can cite the reference on this datasheet.

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