Anti-CNTF Receptor alpha antibody [MM0190-10L29] (ab89333)

Overview

  • Product nameAnti-CNTF Receptor alpha antibody [MM0190-10L29]
    See all CNTF Receptor alpha primary antibodies
  • Description
    Mouse monoclonal [MM0190-10L29] to CNTF Receptor alpha
  • Tested applicationsSuitable for: Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human protein.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab89333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5µg for 106 cells. ab18414-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/1000. Predicted molecular weight: 41 kDa.

Target

  • FunctionBinds to CNTF. The alpha subunit provides the receptor specificity.
  • Tissue specificityNervous system and skeletal muscle.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ciliary neurotrophic factor receptor alpha antibody
    • Ciliary neurotrophic factor receptor antibody
    • Ciliary neurotrophic factor receptor subunit alpha antibody
    • CNTF receptor subunit alpha antibody
    • CNTFR Alpha antibody
    • CNTFR antibody
    • CNTFR-alpha antibody
    • CNTFR_HUMAN antibody
    see all

Anti-CNTF Receptor alpha antibody [MM0190-10L29] images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4µm) labelling CNTF Receptor alpha with ab89333. Tissue was treated with 15µg/ml Protenase K for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.

  • Overlay histogram showing SH-SY5Y cells stained with ab89333 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89333, 0.5µg/1x106 cells ) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2 (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-CNTF Receptor alpha antibody [MM0190-10L29] (ab89333)

ab89333 has not yet been referenced specifically in any publications.

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