Anti-Centrobin antibody (ab70448)
Key features and details
- Mouse polyclonal to Centrobin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Centrobin antibody -
Description
Mouse polyclonal to Centrobin -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human Centrobin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (AAH21134.1, 1 a.a. ~ 903 a.a).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 135 kDa (predicted molecular weight: 102 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 135 kDa (predicted molecular weight: 102 kDa). |
Target
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Function
Required for centriole duplication. Inhibition of centriole duplication leading to defects in cytokinesis. -
Tissue specificity
Widely expressed (at protein level). Highly expressed in testis. Also expressed in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes. -
Developmental stage
Preferentially incorporated into the newly assembled daughter centriole during centriole assembly at the late G1 or early S phase. Remains in the daughter centrioles throughout the cell cycle. At the next cycle of centriole duplication, its amount on the original daughter centriole eventually decreases. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome > centriole. Centriole-associated, asymmetrically localizes to the daughter centriole. - Information by UniProt
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Database links
- Entrez Gene: 116840 Human
- Omim: 611425 Human
- SwissProt: Q8N137 Human
- Unigene: 348012 Human
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Alternative names
- 9830165K03Rik antibody
- Centrobin antibody
- centrobin, centrosomal BRCA2 interacting protein antibody
see all
Images
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All lanes : Anti-Centrobin antibody (ab70448) at 1/500 dilution
Lane 1 : Centrobin transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
Lane 1 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Lane 2 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 102 kDa
Observed band size: 135 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (22)
ab70448 has been referenced in 22 publications.
- Moraes de Farias K et al. The Isoflavanoid (+)-PTC Regulates Cell-Cycle Progression and Mitotic Spindle Assembly in a Prostate Cancer Cell Line. Chem Biodivers 19:e202200102 (2022). PubMed: 35362194
- Balestra FR et al. TRIM37 prevents formation of centriolar protein assemblies by regulating Centrobin. Elife 10:N/A (2021). PubMed: 33491649
- Kong D & Loncarek J Analyzing Centrioles and Cilia by Expansion Microscopy. Methods Mol Biol 2329:249-263 (2021). PubMed: 34085228
- Chen TY et al. Septin 7 is a centrosomal protein that ensures S phase entry and microtubule nucleation by maintaining the abundance of p150glued. J Cell Physiol 236:2706-2724 (2021). PubMed: 32869310
- Meitinger F et al. TRIM37 prevents formation of condensate-organized ectopic spindle poles to ensure mitotic fidelity. J Cell Biol 220:N/A (2021). PubMed: 33983387