Overview

  • Product name
  • Description
    Mouse polyclonal to CNTROB
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    CNTROB (AAH21134.1, 1 a.a. ~ 903 a.a) full-length human protein.

  • Positive control
    • CNTROB transfected 293T lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab70448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20861312
WB 1/500 - 1/1000. Detects a band of approximately 135 kDa (predicted molecular weight: 102 kDa).

Target

  • Function
    Required for centriole duplication. Inhibition of centriole duplication leading to defects in cytokinesis.
  • Tissue specificity
    Widely expressed (at protein level). Highly expressed in testis. Also expressed in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes.
  • Developmental stage
    Preferentially incorporated into the newly assembled daughter centriole during centriole assembly at the late G1 or early S phase. Remains in the daughter centrioles throughout the cell cycle. At the next cycle of centriole duplication, its amount on the original daughter centriole eventually decreases.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome > centriole. Centriole-associated, asymmetrically localizes to the daughter centriole.
  • Information by UniProt
  • Database links
  • Alternative names
    • 9830165K03Rik antibody
    • Centrobin antibody
    • centrobin, centrosomal BRCA2 interacting protein antibody
    • Centrosomal BRCA2 interacting protein antibody
    • Centrosomal BRCA2-interacting protein antibody
    • CNTRB_HUMAN antibody
    • Cntrob antibody
    • LIP8 antibody
    • LYST interacting protein 8 antibody
    • LYST-interacting protein 8 antibody
    • LYST-interacting protein LIP8 antibody
    • PP1221 antibody
    • RGD1307488 antibody
    • RP23 26L6.9 antibody
    see all

Images

  • All lanes : Anti-CNTROB antibody (ab70448) at 1/500 dilution

    Lane 1 : CNTROB transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 102 kDa
    Observed band size : 135 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:

See all 10 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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