Anti-COASY (phospho S182) antibody (ab45994)


  • Product name
    Anti-COASY (phospho S182) antibody
    See all COASY primary antibodies
  • Description
    Rabbit polyclonal to COASY (phospho S182)
  • Specificity
    This antibody is specific for phospho S182 COASY
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human COASY, phosphorylated at S182.

    (Peptide available as ab46197.)

  • Positive control
    • HEK293 cells transiently transfected with wt CoA synthase (COASY)



Our Abpromise guarantee covers the use of ab45994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    WB: Use at an assay dependent dilution. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa) in HEK293 cells transiently transfected with CoA synthase

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation.
    • Tissue specificity
      Expressed in all tissues examined including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocyte. Lowest expression in peripheral blood leukocytes and highest in kidney and liver. Isoform 2 is expressed mainly in the brain.
    • Pathway
      Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 4/5.
      Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5.
    • Sequence similarities
      In the central section; belongs to the eukaryotic coaD family.
      Contains 1 DPCK (dephospho-CoA kinase) domain.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • Bifunctional coenzyme A synthase antibody
      • Bifunctional phosphopantetheine adenylyl transferase/dephospho CoA kinase antibody
      • CoA synthase antibody
      • COASY antibody
      • COASY_HUMAN antibody
      • Coenzyme A synthase antibody
      • Dephospho-CoA kinase antibody
      • Dephospho-CoA pyrophosphorylase antibody
      • Dephosphocoenzyme A kinase antibody
      • DPCK antibody
      • DPCOAK antibody
      • NBP antibody
      • Nucleotide binding protein antibody
      • Pantetheine-phosphate adenylyltransferase antibody
      • Phosphopantetheine adenylyltransferase / dephosphocoenzyme A kinase antibody
      • pOV 2 antibody
      • POV-2 antibody
      • POV2 antibody
      • PPAT antibody
      • UKR1 antibody
      see all

    Anti-COASY (phospho S182) antibody images

    • Predicted band size : 62 kDa

      Western blot analysis of total cell extracts from HEK293 cells transiently transfected with wt and S182A mutant of CoA synthase (CoAsy). This shows that ab45994 specifically recognize CoA synthase phosphorylated at S182. The bottom panel shows the positive loading control b-actin

    References for Anti-COASY (phospho S182) antibody (ab45994)

    ab45994 has not yet been referenced specifically in any publications.

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