Overview

  • Product nameAnti-COBLL1 antibody
    See all COBLL1 primary antibodies
  • Description
    Rabbit polyclonal to COBLL1
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • HepG2 cell lysate. Kidney tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1:312500.

IHC-P Use a concentration of 4 - 8 µg/ml.

Target

Anti-COBLL1 antibody images

  • Anti-COBLL1 antibody (ab64465) at 0.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 132 kDa
    Observed band size : 132 kDa
    Additional bands at : 42 kDa. We are unsure as to the identity of these extra bands.
  • ab64465 at 4µg/ml staining COBLL1 in human kidney tissue, epithelial cells of renal tubule (indicated by arrows)
  • ab64465 staining COBLL1 in human bladder cancer tissue by Immunohistochemistry (formaldehyde fixed sections). Tissue was fixed with formaldehyde, a heat mediated antigen retrieval step was performed using citrate buffer. Tissue was blocked with 1% BSA for 30 minutes at room temperature followed by incubation with the primary antibody, at a 1/80 dilution, for 1 hour. A HRP-conjugated goat anti-rabbit polyclonal was used as secondary antibody un-diluted.
    Note that the protein localizes in the cytoplasm.

    See Abreview

References for Anti-COBLL1 antibody (ab64465)

ab64465 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (bladder cancer)
Specification bladder cancer
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Dr. Karin Birkenkamp-Demtroeder

Verified customer

Submitted Apr 29 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"