The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/100 - 1/500.
Epitope exposure with citrate buffer will enhance staining.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceCOBRA1 is an essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex causes transcriptional pausing and is counteracted by the P-TEFb kinase complex. It may be able to induce chromatin unfolding.
ab84984 at 1/250 dilution staining COBRA in human breast carcinoma by Immunofluorescence, Formalin-fixed, Paraffin-embedded tissue. Detection: Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.