Overview

  • Product name
  • Description
    Rabbit polyclonal to COCH
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human COCH aa 398-428 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: O43405

  • Positive control
    • Human skeletal muscle; HeLa cells; COCH-transfected 293 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab171410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 59 kDa.

Target

  • Function
    Plays a role in the control of cell shape and motility in the trabecular meshwork.
  • Tissue specificity
    Expressed in inner ear structures; the cochlea and the vestibule.
  • Involvement in disease
    Defects in COCH are the cause of deafness autosomal dominant type 9 (DFNA9) [MIM:601369]. DFNA9 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. DFNA9 is characterized by onset in the fourth or fifth decade of life and initially involves the high frequencies. Deafness is progressive and usually complete by the sixth decade. In addition to cochlear involvement, DFNA9 patients also exhibit a spectrum of vestibular dysfunctions. Penetrance of the vestibular symptoms is often incomplete, and some patients are minimally affected, whereas others suffer from severe balance disturbances and episodes of vertigo. Affected individuals have mucopolysaccharide depositions in the channels of the cochlear and vestibular nerves. These depositions apparently cause strangulation and degeneration of dendritic fibers.
  • Sequence similarities
    Contains 1 LCCL domain.
    Contains 2 VWFA domains.
  • Post-translational
    modifications
    N-glycosylated.
    A 50 kDa form is created by proteolytic cleavage.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Coagulation factor C homolog, cochlin (Limulus polyphemus) antibody
    • COCH antibody
    • COCH-5B2 antibody
    • COCH_HUMAN antibody
    • COCH5B2 antibody
    • Cochlin antibody
    • DFNA9 antibody
    see all

Images

  • All lanes : Anti-COCH antibody (ab171410) at 1/100 dilution

    Lane 1 : Non-transfected 293 cell lysate
    Lane 2 : COCH-transfected 293 cell lysate

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 59 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skeletal muscle, labeling COCH with ab171410 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of HeLa cells labeling COCH with ab171410 at 1/10 dilution (right histogram), compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab171410 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Olfactory bulb)
Specification
Olfactory bulb
Username

Abcam user community

Verified customer

Submitted Jan 27 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Olfactory bulb)
Antigen retrieval step
None
Permeabilization
Yes - TritonX
Specification
Olfactory bulb
Blocking step
NGS as blocking agent for 6 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 05 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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