Overview

  • Product name
  • Description
    Rabbit polyclonal to CoCoA
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 202-496 of Human CoCoA (Uniprot ID: Q9P1Z2).

  • Positive control
    • HeLaS3 whole cell lysaate; Human C2C12 xenograft tissue; HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab154731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 77 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance
    Functions as a coactivator for aryl hydrocarbon and nuclear receptors (NR). Recruited to promoters through its contact with the N terminal basic helix-loop-helix-Per-Arnt-Sim (PAS) domain of transcription factors or coactivators, such as NCOA2. During ER-activation acts synergistically in combination with other NCOA2-binding proteins, such as EP300, CREBBP and CARM1. Involved in the transcriptional activation of target genes in the Wnt/CTNNB1 pathway. Functions as a secondary coactivator in LEF1 mediated transcriptional activation via its interaction with CTNNB1. Coactivator function for nuclear receptors and LEF1/CTNNB1 involves differential utilization of two different activation regions.
  • Cellular localization
    Cytoplasm. Nucleus. Note: shuttles between nucleus and cytoplasm.
  • Database links
  • Alternative names
    • Calcium binding and coiled coil domain 1 antibody
    • CALCOCO1 antibody
    • Calphoglin antibody
    • Coiled coil coactivator protein antibody
    • Coiled coil leucine zipper coactivator 1 antibody
    • Coiled coil transcriptional coactivator antibody
    • Inorganic pyrophosphatase activator antibody
    • KIAA1536 antibody
    • PP13275 antibody
    • Sarcoma antigen NY-SAR-3 antibody
    see all

Images

  • Anti-CoCoA antibody (ab154731) at 1/500 dilution + HeLaS3 whole cell lysate at 30 µg

    Predicted band size: 77 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells (ice-cold; 5 mins) labeling CoCoA with ab154731 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human C2C12 xenograft tissue labeling CoCoA with ab154731 at 1/100 dilution.

References

ab154731 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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