Overview

  • Product nameAnti-CoCoA antibody
    See all CoCoA primary antibodies
  • Description
    Rabbit polyclonal to CoCoA
  • Tested applicationsSuitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1 and 50 of human CoCoA (NP_065949.1)

  • Positive control
    • Whole cell lysate from MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab70563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notesIs unsuitable for WB.
  • Target

    • RelevanceFunctions as a coactivator for aryl hydrocarbon and nuclear receptors (NR). Recruited to promoters through its contact with the N terminal basic helix-loop-helix-Per-Arnt-Sim (PAS) domain of transcription factors or coactivators, such as NCOA2. During ER-activation acts synergistically in combination with other NCOA2-binding proteins, such as EP300, CREBBP and CARM1. Involved in the transcriptional activation of target genes in the Wnt/CTNNB1 pathway. Functions as a secondary coactivator in LEF1 mediated transcriptional activation via its interaction with CTNNB1. Coactivator function for nuclear receptors and LEF1/CTNNB1 involves differential utilization of two different activation regions.
    • Cellular localizationCytoplasm. Nucleus. Note: shuttles between nucleus and cytoplasm.
    • Database links
    • Alternative names
      • Calcium binding and coiled coil domain 1 antibody
      • CALCOCO1 antibody
      • Calphoglin antibody
      • Coiled coil coactivator protein antibody
      • Coiled coil leucine zipper coactivator 1 antibody
      • Coiled coil transcriptional coactivator antibody
      • Inorganic pyrophosphatase activator antibody
      • KIAA1536 antibody
      • PP13275 antibody
      • Sarcoma antigen NY-SAR-3 antibody
      see all

    Anti-CoCoA antibody images

    • Detection of human CoCoA by Immunoprecipitation in whole cell lysate from MCF7 cells (1 mg, 20% of immunoprecipitate loaded/lane), using ab70563 at 3 µg/mg of lysate (lane 1).
      CoCoA was also immunoprecipitated using another CoCoA antibody (ab70564) which recognizes a downstream epitope (lane 2).
      Lane 3: Control IgG immunoprecipitate.
      Subsequent blotting of immunoprecipitate for the detection of CoCoA was performed using 1µg/ml of the other antibody (ab70564) and 50µg of lysate. Detection was by chemiluminescence with an exposure time of 30 seconds.

    References for Anti-CoCoA antibody (ab70563)

    ab70563 has not yet been referenced specifically in any publications.

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