Overview

  • Product nameAnti-CoCoA antibody
    See all CoCoA primary antibodies
  • Description
    Rabbit polyclonal to CoCoA
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 650 and the C terminus (residue 691) of human CoCoA (NP_065949.1)

  • Positive control
    • Whole cell lysate from: HeLa, 293T, NIH3T3, Ramos and MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab70564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 77 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • RelevanceFunctions as a coactivator for aryl hydrocarbon and nuclear receptors (NR). Recruited to promoters through its contact with the N terminal basic helix-loop-helix-Per-Arnt-Sim (PAS) domain of transcription factors or coactivators, such as NCOA2. During ER-activation acts synergistically in combination with other NCOA2-binding proteins, such as EP300, CREBBP and CARM1. Involved in the transcriptional activation of target genes in the Wnt/CTNNB1 pathway. Functions as a secondary coactivator in LEF1 mediated transcriptional activation via its interaction with CTNNB1. Coactivator function for nuclear receptors and LEF1/CTNNB1 involves differential utilization of two different activation regions.
  • Cellular localizationCytoplasm. Nucleus. Note: shuttles between nucleus and cytoplasm.
  • Database links
  • Alternative names
    • Calcium binding and coiled coil domain 1 antibody
    • CALCOCO1 antibody
    • Calphoglin antibody
    • Coiled coil coactivator protein antibody
    • Coiled coil leucine zipper coactivator 1 antibody
    • Coiled coil transcriptional coactivator antibody
    • Inorganic pyrophosphatase activator antibody
    • KIAA1536 antibody
    • PP13275 antibody
    • Sarcoma antigen NY-SAR-3 antibody
    see all

Anti-CoCoA antibody images

  • All lanes : Anti-CoCoA antibody (ab70564) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : NIH3T3 whole cell lysate
    Lane 4 : Ramos whole cell lysate
    Lane 5 : MCF7 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 77 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • Detection of human CoCoA by Immunoprecipitation in whole cell lysate from MCF7 cells (1 mg, 20% of immunoprecipitate loaded/lane), using ab70564 at 3 µg/mg of lysate (lane 2).
    CoCoA was also immunoprecipitated using another CoCoA antibody (ab70563) which recognizes an upstream epitope (lane 1).
    Lane 3: Control IgG immunoprecipitate.
    Subsequent blotting of immunoprecipitate for the detection of CoCoA was performed using 1µg/ml of ab70564 and 50µg of lysate.
    Detection was by chemiluminescence with an exposure time of 30 seconds.

References for Anti-CoCoA antibody (ab70564)

This product has been referenced in:

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab70564.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"