Overview

  • Product name
  • Description
    Goat polyclonal to Cofilin 2
  • Specificity
    This antibody is expected to recognise both reported isoforms of cofilin 2.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Chicken, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    DIKDRSTLGEK

    , corresponding to C terminal amino acids 142/152 of Human Cofilin 2

  • Positive control
    • Human muscle lysate. Lysate prepared from HEK293 cells over-expressing Cofilin 2.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab39985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).

Target

  • Function
    Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
  • Tissue specificity
    Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues.
  • Involvement in disease
    Defects in CFL2 are the cause of nemaline myopathy type 7 (NEM7) [MIM:610687]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 7 presents at birth with hypotonia and generalized weakness. Major motor milestones are delayed, but independent ambulation is achieved.
  • Sequence similarities
    Belongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • Post-translational
    modifications
    The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.
  • Cellular localization
    Nucleus matrix. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • CFL 2 antibody
    • CFL2 antibody
    • COF2_HUMAN antibody
    • Cofilin 2 muscle antibody
    • Cofilin antibody
    • Cofilin muscle antibody
    • Cofilin muscle isoform antibody
    • Cofilin-2 antibody
    • Cofilin2 antibody
    • muscle isoform antibody
    • NEM 7 antibody
    • NEM7 antibody
    see all

Images

  • Anti-Cofilin 2 antibody (ab39985) at 0.003 µg/ml (for 1 hour) + Human Muscle lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 18 kDa
    Observed band size : 18 kDa
  • Anti-Cofilin 2 antibody (ab39985) at 0.03 µg/ml + lysate prepared from HEK293 cells over-expressing Cofilin 2

    Predicted band size : 18 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Sigloch FC  et al. miR-200c dampens cancer cell migration via regulation of protein kinase A subunits. Oncotarget 6:23874-89 (2015). WB . Read more (PubMed: 26203557) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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