Overview

  • Product nameAnti-Cofilin 2 antibody
    See all Cofilin 2 primary antibodies
  • Description
    Mouse monoclonal to Cofilin 2
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide Recombinant fragment, corresponding to amino acids 57-167 of Human Cofilin 2

Properties

Applications

Our Abpromise guarantee covers the use of ab54536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 19 kDa.
IHC-P Use a concentration of 8 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionControls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
  • Tissue specificityIsoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues.
  • Involvement in diseaseDefects in CFL2 are the cause of nemaline myopathy type 7 (NEM7) [MIM:610687]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 7 presents at birth with hypotonia and generalized weakness. Major motor milestones are delayed, but independent ambulation is achieved.
  • Sequence similaritiesBelongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • Post-translational
    modifications
    The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.
  • Cellular localizationNucleus matrix. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • CFL 2 antibody
    • CFL2 antibody
    • COF2_HUMAN antibody
    • Cofilin 2 muscle antibody
    • Cofilin antibody
    • Cofilin muscle antibody
    • Cofilin muscle isoform antibody
    • Cofilin-2 antibody
    • Cofilin2 antibody
    • muscle isoform antibody
    • NEM 7 antibody
    • NEM7 antibody
    see all

Anti-Cofilin 2 antibody images

  • Cofilin 2 antibody (ab54536) used in immunohistochemistry at 8ug/ml on formalin fixed and paraffin embedded human skeletal muscle.


  • Predicted band size : 19 kDa
    Cofilin 2 antibody (ab54536) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab54536 at 10 µg/ml staining Cofillin 2 in human Hela cells by Immunocytochemistry / Immunofluorescence.

References for Anti-Cofilin 2 antibody (ab54536)

ab54536 has not yet been referenced specifically in any publications.

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