• Product name
  • Description
    Rabbit polyclonal to Cofilin 2
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 92 and 152 of Cofilin 2.

  • Positive control
    • WB: 293T, A431, HeLaS3, HepG2, MOLT4 or Raji cell lysate; ICC/IF: HeLa cells; IHC-P: gastric carcinoma tissue.



Our Abpromise guarantee covers the use of ab96678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 18 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/200.


  • Function
    Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
  • Tissue specificity
    Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues.
  • Involvement in disease
    Defects in CFL2 are the cause of nemaline myopathy type 7 (NEM7) [MIM:610687]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 7 presents at birth with hypotonia and generalized weakness. Major motor milestones are delayed, but independent ambulation is achieved.
  • Sequence similarities
    Belongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • Post-translational
    The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.
  • Cellular localization
    Nucleus matrix. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • CFL 2 antibody
    • CFL2 antibody
    • COF2_HUMAN antibody
    • Cofilin 2 muscle antibody
    • Cofilin antibody
    • Cofilin muscle antibody
    • Cofilin muscle isoform antibody
    • Cofilin-2 antibody
    • Cofilin2 antibody
    • muscle isoform antibody
    • NEM 7 antibody
    • NEM7 antibody
    see all


  • Anti-Cofilin 2 antibody (ab96678) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 18 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96678 at 1/200 dilution.
  • Immunohistochemistry analysis of paraffin-embedded gastric carcinoma, using ab96678 at 1/100 dilution.


This product has been referenced in:
  • Chapnik E  et al. miR-142 orchestrates a network of actin cytoskeleton regulators during megakaryopoiesis. Elife 3:e01964 (2014). WB . Read more (PubMed: 24859754) »

See 1 Publication for this product

Customer reviews and Q&As

The immunogen is found within amino acids 92 to 152. I asked the lab to align the immunogen used to create ab96678 and the amino acid sequence of Cofilin 1. The percentage of mismatches between them is about 30%. At most there will be minimal cross rea...

Read More

BLASTing the immunogen sequence of ab96678 against Cofilin 1, no significant similarity was found. So it is unlikely this antibody is detecting Cofilin 1. I hope this is helpful. Please contact us again if you have any further questions.


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