Overview

  • Product nameAnti-Cofilin 2 antibody
    See all Cofilin 2 primary antibodies
  • Description
    Rabbit polyclonal to Cofilin 2
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 92 and 152 of Cofilin 2.

  • Positive control
    • WB: 293T, A431, HeLaS3, HepG2, MOLT4 or Raji cell lysate; ICC/IF: HeLa cells; IHC-P: gastric carcinoma tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab96678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 18 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/200.

Target

  • FunctionControls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
  • Tissue specificityIsoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues.
  • Involvement in diseaseDefects in CFL2 are the cause of nemaline myopathy type 7 (NEM7) [MIM:610687]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 7 presents at birth with hypotonia and generalized weakness. Major motor milestones are delayed, but independent ambulation is achieved.
  • Sequence similaritiesBelongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • Post-translational
    modifications
    The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.
  • Cellular localizationNucleus matrix. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • CFL 2 antibody
    • CFL2 antibody
    • COF2_HUMAN antibody
    • Cofilin 2 muscle antibody
    • Cofilin antibody
    • Cofilin muscle antibody
    • Cofilin muscle isoform antibody
    • Cofilin-2 antibody
    • Cofilin2 antibody
    • muscle isoform antibody
    • NEM 7 antibody
    • NEM7 antibody
    see all

Anti-Cofilin 2 antibody images

  • Anti-Cofilin 2 antibody (ab96678) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 18 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96678 at 1/200 dilution.
  • Immunohistochemistry analysis of paraffin-embedded gastric carcinoma, using ab96678 at 1/100 dilution.

References for Anti-Cofilin 2 antibody (ab96678)

ab96678 has not yet been referenced specifically in any publications.

Product Wall

The immunogen is found within amino acids 92 to 152. I asked the lab to align the immunogen used to create ab96678 and the amino acid sequence of Cofilin 1. The percentage of mismatches between them is about 30%. At most there will be minimal cross rea...

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BLASTing the immunogen sequence of ab96678 against Cofilin 1, no significant similarity was found. So it is unlikely this antibody is detecting Cofilin 1. I hope this is helpful. Please contact us again if you have any further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"