Anti-Cofilin 2 antibody - C-terminal (ab179935)

Overview

  • Product name
    Anti-Cofilin 2 antibody - C-terminal
    See all Cofilin 2 primary antibodies
  • Description
    Goat polyclonal to Cofilin 2 - C-terminal
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Hamster, Cow, Dog, Pig, Monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human Cofilin 2 aa 142-152 (C terminal) (Cysteine residue).
    Sequence:

    DIKDRSTLGEK


    Database link: Q9Y281

  • Positive control
    • Human muscle lysate; Human heart tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, 99% Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab179935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 19 kDa.
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
  • Tissue specificity
    Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues.
  • Involvement in disease
    Defects in CFL2 are the cause of nemaline myopathy type 7 (NEM7) [MIM:610687]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 7 presents at birth with hypotonia and generalized weakness. Major motor milestones are delayed, but independent ambulation is achieved.
  • Sequence similarities
    Belongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • Post-translational
    modifications
    The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal.
  • Cellular localization
    Nucleus matrix. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • CFL 2 antibody
    • CFL2 antibody
    • COF2_HUMAN antibody
    • Cofilin 2 muscle antibody
    • Cofilin antibody
    • Cofilin muscle antibody
    • Cofilin muscle isoform antibody
    • Cofilin-2 antibody
    • Cofilin2 antibody
    • muscle isoform antibody
    • NEM 7 antibody
    • NEM7 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin embedded Human heart tissue labeling Cofilin 2 with ab179935 at 3.75 µg/ml.

  • Anti-Cofilin 2 antibody - C-terminal (ab179935) at 0.03 µg/ml + Human muscle lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 19 kDa


    Exposure time: 1 hour

References

ab179935 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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