Overview

  • Product nameAnti-Cofilin antibody
    See all Cofilin primary antibodies
  • Description
    Rabbit polyclonal to Cofilin
  • Tested applicationsSuitable for: IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Dog, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to amino acids 154-166 of human cofilin with N-terminal cysteine added, conjugated to maleimide-activated keyhole limpet hemocyanin (KLH). The corresponding immunizing sequence is identical in pig and rat non-muscle cofilin and differs by three amino acids from that of human and chicken muscle cofilin.

  • General notesThe corresponding immunizing sequence is identical in pig and rat non-muscle cofilin and differs by three amino acids from that of human and chicken muscle cofilin.

Properties

Applications

Our Abpromise guarantee covers the use of ab11062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/10000. Predicted molecular weight: 19 kDa.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionControls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
  • Tissue specificityWidely distributed in various tissues.
  • Sequence similaritiesBelongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • Post-translational
    modifications
    Phosphorylated on Ser-3 in resting cells.
  • Cellular localizationNucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
  • Information by UniProt
  • Database links
  • Alternative names
    • 18 kDa phosphoprotein antibody
    • CFL 1 antibody
    • CFL antibody
    • CFL1 antibody
    • COF1_HUMAN antibody
    • Cofilin 1 antibody
    • Cofilin 1 non muscle antibody
    • Cofilin antibody
    • Cofilin non muscle isoform antibody
    • Cofilin-1 antibody
    • epididymis secretory protein Li 15 antibody
    • HEL-S-15 antibody
    • non-muscle isoform antibody
    • p18 antibody
    see all

Anti-Cofilin antibody images

  • ICC/IF image of ab11062 stained human HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11062, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.

References for Anti-Cofilin antibody (ab11062)

This product has been referenced in:
  • Serrano-Pertierra E  et al. NKG2D- and CD28-mediated costimulation regulate CD8+ T cell chemotaxis through different mechanisms: the role of Cdc42/N-WASp. J Leukoc Biol 95:487-95 (2014). Read more (PubMed: 24168855) »
  • De Filippis B  et al. Pharmacological Stimulation of the Brain Serotonin Receptor 7 as a Novel Therapeutic Approach for Rett Syndrome. Neuropsychopharmacology N/A:N/A (2014). Read more (PubMed: 24809912) »

See all 12 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Rat Tissue lysate - whole (Brain)
Total protein in input 1000 µg
Specification Brain
Immuno-precipitation step Protein G
Username

Abcam user community

Verified customer

Submitted Sep 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (pancreatic cancer cell line)
Loading amount 20 µg
Specification pancreatic cancer cell line
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Hannah Whiteman

Verified customer

Submitted Jul 04 2008

Thank you for your phone call and I apologize for the shortage of ab11062 that you received. We are sending you a free of charge replacement vial of this antibody; it is on order reference# 111878. As the item is currently out of stock the estimated de...

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Thank you for your enquiry. When ab11062 was tested in IF with mouse NIH/3T3 fibroblasts, antigen retrieval was not performed. The cells were fixed with 3-4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Ab11062 was used at a dilution of 1...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HUVEC)
Specification HUVEC
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5 %
Username

Abcam user community

Verified customer

Submitted Aug 15 2005

Thank you for your enquiry and your interest in our products. We regret to inform you that the cross-reactivity of ab11062 (cofilin 1) with cofilin 2 has not been tested yet. On the basis of poor sequence homology between human Cofilin 1 and Co...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"