• Product nameAnti-Cofilin antibody
    See all Cofilin primary antibodies
  • Description
    Mouse monoclonal to Cofilin
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-167 of Human Cofilin



Our Abpromise guarantee covers the use of ab54532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 18 kDa.
IHC-P Use a concentration of 1.5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.


  • FunctionControls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
  • Tissue specificityWidely distributed in various tissues.
  • Sequence similaritiesBelongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • Post-translational
    Phosphorylated on Ser-3 in resting cells.
  • Cellular localizationNucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
  • Information by UniProt
  • Database links
  • Alternative names
    • 18 kDa phosphoprotein antibody
    • CFL 1 antibody
    • CFL antibody
    • CFL1 antibody
    • COF1_HUMAN antibody
    • Cofilin 1 antibody
    • Cofilin 1 non muscle antibody
    • Cofilin antibody
    • Cofilin non muscle isoform antibody
    • Cofilin-1 antibody
    • epididymis secretory protein Li 15 antibody
    • HEL-S-15 antibody
    • non-muscle isoform antibody
    • p18 antibody
    see all

Anti-Cofilin antibody images

  • Predicted band size : 18 kDa
    Cofilin antibody (ab54532) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab54532 at 1.5 µg/ml staining human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

  • ab54532 at 10 µg/ml staining Cofilin in human Hela cells by Immunocytochemistry / Immunofluorescence.

  • Anti-Cofilin antibody (ab54532) at 1 µg/ml + Cell lysate prepared from mouse NIH/3T3 cells

    Predicted band size : 18 kDa
  • Anti-Cofilin antibody (ab54532) at 1 µg/ml + Cell lysates prepared from rat PC12 cells.

    Predicted band size : 18 kDa

References for Anti-Cofilin antibody (ab54532)

This product has been referenced in:
  • Eshraghi M  et al. Effect of genetic background on the phenotype of the Smn2B/- mouse model of spinal muscular atrophy. Hum Mol Genet N/A:N/A (2016). Read more (PubMed: 27538421) »
  • Calabrese B  et al. Activity-Dependent Dendritic Spine Shrinkage and Growth Involve Downregulation of Cofilin via Distinct Mechanisms. PLoS One 9:e94787 (2014). ICC/IF ; Rat . Read more (PubMed: 24740405) »

See all 9 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Rat Cell lysate - whole cell (PC12, rat pheochromacytoma)
Total protein in input 1000 µg
Specification PC12, rat pheochromacytoma
Immuno-precipitation step Protein G

Abcam user community

Verified customer

Submitted Oct 31 2012

Thank you for your reply. I am very pleased to hear you would like to accept our offer and test ab54532 in IP.

Please place your order for ab54532 with the following discount code. This first order will be invoiced at full price:


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Thank you very much for your reply. I am pleased to hear you are interested in the testing discount offer.

I can offer a discount off a future purchase if you buy ab54532 now, test it in IP and submit feedback to us in the form of an Abrevie...

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Thank you for contacting us and for your interest in our products.

As far as we are aware, ab54532 has never been tested in IP. All tested applications covered by our 6 month guarantee are specified on our datasheets, and these are updated a...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from ab54532. The details you have kindly provided will provide us with vital information for our monitoring of product quality

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Merci pour votre appel et votre patience.
Le laboratoire vient de me confirmer que ils n'ont pas fait un "epitope mapping" sur cet anticorps, et donc que l'épitope de cet anticorps n'est pas connue. Effectivement, l'immunogène était toute la proté...

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Thank you for contacting us. I am sorry that this new vial of antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab75510. To check the status of the order please contact our Customer...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab75510. This product is currently out of stock. You should expect to...

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Thank you for your enquiry. I am sorry this vial of ab54532 is giving you trouble. Here is a link to another anti-Cofilin antibody you may be interested in: http://www.abcam.com/Cofilin-antibody-ab29038.html As a reminder, please check the datasheet fo...

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