Anti-Cofilin antibody [AF2C2] (ab75510)

Overview

  • Product nameAnti-Cofilin antibody [AF2C2]
    See all Cofilin primary antibodies
  • Description
    Mouse monoclonal [AF2C2] to Cofilin
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length Cofilin protein (Human) purified from E.coli (His-Cofilin)

  • Positive control
    • Jurkat, Raji, SK-N-MC, PC12 and NIH3T3 cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab75510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
ELISA 1/2000.

Target

  • FunctionControls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
  • Tissue specificityWidely distributed in various tissues.
  • Sequence similaritiesBelongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • Post-translational
    modifications
    Phosphorylated on Ser-3 in resting cells.
  • Cellular localizationNucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
  • Information by UniProt
  • Database links
  • Alternative names
    • 18 kDa phosphoprotein antibody
    • CFL 1 antibody
    • CFL antibody
    • CFL1 antibody
    • COF1_HUMAN antibody
    • Cofilin 1 antibody
    • Cofilin 1 non muscle antibody
    • Cofilin antibody
    • Cofilin non muscle isoform antibody
    • Cofilin-1 antibody
    • epididymis secretory protein Li 15 antibody
    • HEL-S-15 antibody
    • non-muscle isoform antibody
    • p18 antibody
    see all

Anti-Cofilin antibody [AF2C2] images

  • All lanes : Anti-Cofilin antibody [AF2C2] (ab75510) at 1/2000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : SK-N-MC cell lysate
    Lane 4 : PC12 cell lysate
    Lane 5 : NIH3T3 cell lysate


    Predicted band size : 18 kDa
    Observed band size : 18 kDa

References for Anti-Cofilin antibody [AF2C2] (ab75510)

ab75510 has not yet been referenced specifically in any publications.

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The exact epitope that this antibody recognizes has not yet been identified. Since ab75510 is a monoclonal antibody raised against the full length human cofilin protein, the epitope could lie an...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab75510. To check the status of the order please contact our Customer...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"