Anti-Cofilin antibody [AF2C2] (ab75510)

Overview

  • Product name
    Anti-Cofilin antibody [AF2C2]
    See all Cofilin primary antibodies
  • Description
    Mouse monoclonal [AF2C2] to Cofilin
  • Tested applications
    Suitable for: ChIP, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length Cofilin protein (Human) purified from E.coli (His-Cofilin)

  • Positive control
    • Jurkat, Raji, SK-N-MC, PC12 and NIH3T3 cell lysates
  • General notes

    This product was changed from ascites to tissue culture supernatant on 18th September 2017. Lot numbers higher than GR166582 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab75510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
WB Use a concentration of 4 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
ELISA Use at an assay dependent concentration.

Target

  • Function
    Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
  • Tissue specificity
    Widely distributed in various tissues.
  • Sequence similarities
    Belongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • Post-translational
    modifications
    Phosphorylated on Ser-3 in resting cells.
  • Cellular localization
    Nucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
  • Information by UniProt
  • Database links
  • Alternative names
    • 18 kDa phosphoprotein antibody
    • CFL 1 antibody
    • CFL antibody
    • CFL1 antibody
    • COF1_HUMAN antibody
    • Cofilin 1 antibody
    • Cofilin 1 non muscle antibody
    • Cofilin antibody
    • Cofilin non muscle isoform antibody
    • Cofilin-1 antibody
    • epididymis secretory protein Li 15 antibody
    • HEL-S-15 antibody
    • non-muscle isoform antibody
    • p18 antibody
    see all

Anti-Cofilin antibody [AF2C2] images

  • All lanes : Anti-Cofilin antibody [AF2C2] (ab75510) at 1/2000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : SK-N-MC cell lysate
    Lane 4 : PC12 cell lysate
    Lane 5 : NIH3T3 cell lysate


    Predicted band size : 18 kDa
    Observed band size : 18 kDa

References for Anti-Cofilin antibody [AF2C2] (ab75510)

ab75510 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us with your question.

The exact epitope that this antibody recognizes has not yet been identified. Since ab75510 is a monoclonal antibody raised against the full length human cofilin protein, the epitope could lie an...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab75510. To check the status of the order please contact our Customer...

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