Recombinant Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6375] to Cofilin - Loading Control
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Cofilin antibody [EPR6375] - Loading Control
See all Cofilin-1 primary antibodies -
Description
Rabbit monoclonal [EPR6375] to Cofilin - Loading Control -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Cofilin aa 1-100. The exact sequence is proprietary.
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Positive control
- 293T and Caco-2 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6375 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Predicted molecular weight: 19 kDa.
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
1/1000. Predicted molecular weight: 19 kDa. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. -
Tissue specificity
Widely distributed in various tissues. -
Sequence similarities
Belongs to the actin-binding proteins ADF family.
Contains 1 ADF-H domain. -
Post-translational
modificationsPhosphorylated on Ser-3 in resting cells. -
Cellular localization
Nucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide. - Information by UniProt
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Database links
- Entrez Gene: 1072 Human
- Entrez Gene: 12631 Mouse
- Entrez Gene: 29271 Rat
- Omim: 601442 Human
- SwissProt: P23528 Human
- SwissProt: P18760 Mouse
- SwissProt: P45592 Rat
- Unigene: 170622 Human
see all -
Alternative names
- 18 kDa phosphoprotein antibody
- CFL 1 antibody
- CFL antibody
see all
Images
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All lanes : Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/1000 dilution (purified)
Lane 1 : NIH/3T3 cell lysate
Lane 2 : C6 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDaBlocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
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Immunofluorescent analysis of 4% paraformaldehyde-fixed,0.1 % Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lines labeling Cofilin with ab124979 at 1/500 dilution,followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
The nuclear counterstain is DAPI (blue).
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Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/1000 dilution (purified) + HEK293 cell lysate at 10 µg
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDaBlocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
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Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/5000 dilution (purified) + Caco-2 cell lysate at 10 µg
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDaBlocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
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All lanes : Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/1000 dilution (unpurified)
Lane 1 : 293T cell lysate
Lane 2 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 19 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab124979 has been referenced in 9 publications.
- Wang Y et al. Comprehensive Surfaceome Profiling to Identify and Validate Novel Cell-Surface Targets in Osteosarcoma. Mol Cancer Ther 21:903-913 (2022). PubMed: 35312779
- Zhang L et al. Nujiangexanthone A Inhibits Hepatocellular Carcinoma Metastasis via Down Regulation of Cofilin 1. Front Cell Dev Biol 9:644716 (2021). PubMed: 33791303
- Jin L et al. ETS1-activated SNHG10 exerts oncogenic functions in glioma via targeting miR-532-3p/FBXL19 axis. Cancer Cell Int 20:589 (2020). PubMed: 33298070
- Yang W et al. Co-overexpression of VEGF and GDNF in adipose-derived stem cells optimizes therapeutic effect in neurogenic erectile dysfunction model. Cell Prolif 53:e12756 (2020). PubMed: 31943490
- Zhang M et al. ZEB1-activated LINC01123 accelerates the malignancy in lung adenocarcinoma through NOTCH signaling pathway. Cell Death Dis 11:981 (2020). PubMed: 33191397
- Fei X et al. Eupatilin inhibits glioma proliferation, migration, and invasion by arresting cell cycle at G1/S phase and disrupting the cytoskeletal structure. Cancer Manag Res 11:4781-4796 (2019). PubMed: 31213900
- Kopp S et al. The role of NF?B in spheroid formation of human breast cancer cells cultured on the Random Positioning Machine. Sci Rep 8:921 (2018). WB ; Human . PubMed: 29343717
- Sukma Dewi I et al. Exosomal miR-142-3p is increased during cardiac allograft rejection and augments vascular permeability through down-regulation of endothelial RAB11FIP2 expression. Cardiovasc Res 113:440-452 (2017). PubMed: 28073833
- Xu Z et al. Hepatitis B virus X induces inflammation and cancer in mice liver through dysregulation of cytoskeletal remodeling and lipid metabolism. Oncotarget 7:70559-70574 (2016). PubMed: 27708241