Overview

  • Product nameAnti-Cofilin (phospho S3) antibody
    See all Cofilin primary antibodies
  • Description
    Rabbit polyclonal to Cofilin (phospho S3)
  • SpecificityThis antibody is specific for Cofilin, only when phosphorylated at serine 3.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthesized phosphopeptide derived from human Cofilin around the phosphorylation site of serine 3 (M-A-SP-G-V)

  • Positive control
    • WB: lysate of COLO205 cells serum starved. IHC-P: Human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab47281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionControls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
  • Tissue specificityWidely distributed in various tissues.
  • Sequence similaritiesBelongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • Post-translational
    modifications
    Phosphorylated on Ser-3 in resting cells.
  • Cellular localizationNucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
  • Information by UniProt
  • Database links
  • Alternative names
    • 18 kDa phosphoprotein antibody
    • CFL 1 antibody
    • CFL antibody
    • CFL1 antibody
    • COF1_HUMAN antibody
    • Cofilin 1 antibody
    • Cofilin 1 non muscle antibody
    • Cofilin antibody
    • Cofilin non muscle isoform antibody
    • Cofilin-1 antibody
    • epididymis secretory protein Li 15 antibody
    • HEL-S-15 antibody
    • non-muscle isoform antibody
    • p18 antibody
    see all

Anti-Cofilin (phospho S3) antibody images

  • All lanes : Anti-Cofilin (phospho S3) antibody (ab47281) at 1/500 dilution

    Lane 1 : Extracts from COLO205 cells serum starved
    Lane 2 : Extracts from COLO205 cells

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 18 kDa
    Observed band size : 18 kDa
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47281 at a 1/50 dilution.

    Left image : Untreated

    Right image : Treated with P-Peptide

References for Anti-Cofilin (phospho S3) antibody (ab47281)

This product has been referenced in:
  • Kim JE  et al. LIM kinase-2 induces programmed necrotic neuronal death via dysfunction of DRP1-mediated mitochondrial fission. Cell Death Differ N/A:N/A (2014). WB . Read more (PubMed: 24561342) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Fruit fly (Drosophila melanogaster) Tissue lysate - other (heads (20 heads loaded))
Loading amount 20 cells
Specification heads (20 heads loaded)
Gel Running Conditions Reduced Denaturing (4-20)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Username

Dr. Doris Kretzschmar

Verified customer

Submitted Dec 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"