• Product name
    Anti-Cofilin (phospho S3) antibody
    See all Cofilin primary antibodies
  • Description
    Rabbit polyclonal to Cofilin (phospho S3)
  • Specificity
    This antibody is specific for Cofilin, only when phosphorylated at serine 3.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthesized phosphopeptide derived from human Cofilin around the phosphorylation site of serine 3 (M-A-SP-G-V)

  • Positive control
    • WB: lysate of COLO205 cells serum starved. IHC-P: Human breast carcinoma tissue



Our Abpromise guarantee covers the use of ab47281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.


  • Function
    Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
  • Tissue specificity
    Widely distributed in various tissues.
  • Sequence similarities
    Belongs to the actin-binding proteins ADF family.
    Contains 1 ADF-H domain.
  • Post-translational
    Phosphorylated on Ser-3 in resting cells.
  • Cellular localization
    Nucleus matrix. Cytoplasm > cytoskeleton. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.
  • Information by UniProt
  • Database links
  • Alternative names
    • 18 kDa phosphoprotein antibody
    • CFL 1 antibody
    • CFL antibody
    • CFL1 antibody
    • COF1_HUMAN antibody
    • Cofilin 1 antibody
    • Cofilin 1 non muscle antibody
    • Cofilin antibody
    • Cofilin non muscle isoform antibody
    • Cofilin-1 antibody
    • epididymis secretory protein Li 15 antibody
    • HEL-S-15 antibody
    • non-muscle isoform antibody
    • p18 antibody
    see all

Anti-Cofilin (phospho S3) antibody images

  • All lanes : Anti-Cofilin (phospho S3) antibody (ab47281) at 1/500 dilution

    Lane 1 : Extracts from COLO205 cells serum starved
    Lane 2 : Extracts from COLO205 cells

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 18 kDa
    Observed band size : 18 kDa
    Preparation of positive control: when the cells were at the log phase and 80-90% confluent, the culture media was drained. Cells were washed with fresh basic media (no serum) once. Add fresh basic media to the vessels and starve the cells for 18-24 hours. Drain the cultured medium, then add 20% serum to the vessels for 15 mins.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47281 at a 1/50 dilution.

    Left image : Untreated

    Right image : Treated with P-Peptide

References for Anti-Cofilin (phospho S3) antibody (ab47281)

This product has been referenced in:
  • Tsai CS  et al. Thrombomodulin regulates monocye differentiation via PKCd and ERK1/2 pathway in vitro and in atherosclerotic artery. Sci Rep 6:38421 (2016). WB ; Human . Read more (PubMed: 27910925) »
  • Kim JE  et al. LIM kinase-2 induces programmed necrotic neuronal death via dysfunction of DRP1-mediated mitochondrial fission. Cell Death Differ N/A:N/A (2014). WB . Read more (PubMed: 24561342) »

See all 2 Publications for this product

Product Wall

Western blot
Fruit fly (Drosophila melanogaster) Tissue lysate - other (heads (20 heads loaded))
Loading amount
20 cells
heads (20 heads loaded)
Gel Running Conditions
Reduced Denaturing (4-20)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

Dr. Doris Kretzschmar

Verified customer

Submitted Dec 06 2010


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