For the best experience on the Abcam website please upgrade to a modern browser such as
Rabbit polyclonal to COG3 - N-terminal
Predicted to work with:
Pig, Chimpanzee, Rhesus monkey, Gorilla, Common marmoset
Synthetic peptide within Human COG3 aa 1-50 (N terminal). The exact sequence is proprietary. (EAX08741.1).
HeLa, 293T and Jurkat whole cell lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate pH 7 to 8
Concentration information loading...
Immunogen affinity purified
ab194960 was affinity purified using an epitope specific to COG3 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
1/2000 - 1/10000. Predicted molecular weight: 94 kDa.
Involved in ER-Golgi transport.
Widely expressed with highest levels in pancreas and testis and lowest levels in lung.
Belongs to the COG3 family.
Golgi apparatus > Golgi stack membrane. Associated with the peripheral membrane of cis/medial cisternae.
Information by UniProt
COG complex subunit 3 antibody
Western blot - Anti-COG3 antibody - N-terminal (ab194960)
All lanes :
Anti-COG3 antibody - N-terminal (ab194960) at 0.1 µg/ml
Lane 1 :
HeLa whole cell lysate
Lane 2 :
293T whole cell lysate
Lane 3 :
Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size:
Lysates prepared using NETN lysis buffer.
Immunoprecipitation - Anti-COG3 antibody - N-terminal (ab194960)
Detection of COG3 in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab194960 at 6 µg/mg lysate for IP and at 0.1 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"