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Rabbit polyclonal to COG3 - N-terminal
Predicted to work with:
Pig, Chimpanzee, Rhesus monkey, Gorilla, Common marmoset
Synthetic peptide within Human COG3 aa 1-50 (N terminal). The exact sequence is proprietary. (EAX08741.1).
HeLa, 293T and Jurkat whole cell lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate pH 7 to 8
Concentration information loading...
Immunogen affinity purified
ab194960 was affinity purified using an epitope specific to COG3 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
1/2000 - 1/10000. Predicted molecular weight: 94 kDa.
Involved in ER-Golgi transport.
Widely expressed with highest levels in pancreas and testis and lowest levels in lung.
Belongs to the COG3 family.
Golgi apparatus > Golgi stack membrane. Associated with the peripheral membrane of cis/medial cisternae.
Information by UniProt
COG complex subunit 3 antibody
Western blot - Anti-COG3 antibody - N-terminal (ab194960)
All lanes :
Anti-COG3 antibody - N-terminal (ab194960) at 0.1 µg/ml
Lane 1 :
HeLa whole cell lysate
Lane 2 :
293T whole cell lysate
Lane 3 :
Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique
Predicted band size :
Exposure time :
Lysates prepared using NETN lysis buffer.
Immunoprecipitation - Anti-COG3 antibody - N-terminal (ab194960)
Detection of COG3 in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab194960 at 6 µg/mg lysate for IP and at 0.1 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"