Anti-COG3 antibody - N-terminal (ab194960)

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Product name

Anti-COG3 antibody - N-terminal
See all COG3 primary antibodies
Description

Rabbit polyclonal to COG3 - N-terminal
Host species

Rabbit
Tested applications

Suitable for: IP, WBmore details
Species reactivity

Reacts with: Human
Predicted to work with: Pig, Chimpanzee, Rhesus monkey, Gorilla, Common marmoset
Immunogen

Synthetic peptide within Human COG3 aa 1-50 (N terminal). The exact sequence is proprietary. (EAX08741.1).
Sequence:
MAEAALLLLPEAAAERDAREKLALWDRRPDTTAPLTDRQTDSVLELKAAA

Database link: Q96JB2

Run BLAST with Run BLAST with
Positive control
- HeLa, 293T and Jurkat whole cell lysates.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate

pH 7 to 8
Concentration information loading...
Purity

Immunogen affinity purified
Purification notes

ab194960 was affinity purified using an epitope specific to COG3 immobilized on solid support.
Clonality

Polyclonal
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab194960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IP		Use at 2-10 µg/mg of lysate.
WB		1/2000 - 1/10000. Predicted molecular weight: 94 kDa.

Function

Involved in ER-Golgi transport.
Tissue specificity

Widely expressed with highest levels in pancreas and testis and lowest levels in lung.
Sequence similarities

Belongs to the COG3 family.
Cellular localization

Golgi apparatus > Golgi stack membrane. Associated with the peripheral membrane of cis/medial cisternae.
Target information above from: UniProt accession Q96JB2 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 452579 Chimpanzee
- Entrez Gene: 100408528 Common marmoset
- Entrez Gene: 83548 Human
- Entrez Gene: 100622528 Pig
- Entrez Gene: 702375 Rhesus monkey
- Omim: 606975 Human
- SwissProt: Q96JB2 Human
- Unigene: 507948 Human
Alternative names
- COG complex subunit 3 antibody
- Cog3 antibody
- COG3_HUMAN antibody
- Component of golgi transport complex 3 antibody
- Component of oligomeric Golgi complex 3 antibody
- Conserved oligomeric Golgi complex subunit 3 antibody
- OTTHUMP00000018352 antibody
- p94 antibody
- SEC34 antibody
- SEC34 S. cerevisiae homolog of antibody
- SEC34, S. cerevisiae, homolog of antibody
- Tethering factor SEC34 antibody
- Vesicle docking protein SEC34 homolog antibody
- Vesicle-docking protein SEC34 homolog antibody
see all

Western blot - Anti-COG3 antibody - N-terminal (ab194960)

All lanes : Anti-COG3 antibody - N-terminal (ab194960) at 0.1 µg/ml

Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate

Lysates/proteins at 50 µg per lane.

Developed using the ECL technique.

Predicted band size: 94 kDa

Exposure time: 30 seconds

Lysates prepared using NETN lysis buffer.
Immunoprecipitation - Anti-COG3 antibody - N-terminal (ab194960)

Detection of COG3 in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab194960 at 6 µg/mg lysate for IP and at 0.1 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 30 seconds.

ab194960 has not yet been referenced specifically in any publications.

Publishing research using ab194960? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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