Overview

  • Product name
  • Description
    Rabbit polyclonal to COG4
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 684-733 (TSLVAVELEK VVLKSTFNRL GGLQFDKELR SLIAYLTTVT TWTIRDKFAR) of human COG4 (NP_056201).

  • Positive control
    • Hela cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 89 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

Target

  • Relevance
    COG4 is a component of the conserved oligomeric Golgi (COG) complex which is composed of eight different subunits and is required for normal Golgi morphology and localization. COG4 is required for normal Golgi function.
  • Cellular localization
    Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side.
  • Database links
  • Alternative names
    • CDG2J antibody
    • COD1 antibody
    • COG 4 antibody
    • COG complex subunit 4 antibody
    • Complexed with Dor1p antibody
    • Component of oligomeric golgi complex 4 antibody
    • Conserved oligomeric Golgi complex protein 4 antibody
    • Conserved oligomeric Golgi complex subunit 4 antibody
    • DKFZp586E1519 antibody
    see all

Images

  • Anti-COG4 antibody (ab83479) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 89 kDa
    Observed band size : 89 kDa
    Additional bands at : 50 kDa,>90 kDa. We are unsure as to the identity of these extra bands.

References

ab83479 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab83479.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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