Recombinant
RabMAb

Anti-COG4 antibody [EPR10111] (ab154795)

Overview

  • Product name
    Anti-COG4 antibody [EPR10111]
    See all COG4 primary antibodies
  • Description
    Rabbit monoclonal [EPR10111] to COG4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues at the C terminus of Human COG4 (UniProt Q9H9E3).

  • Positive control
    • HepG2, HeLa and K562 cell lysates; Human heart and kidney tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 89 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/500. Use with paraformaldehyde or methanol fixed cells.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Relevance
      COG4 is a component of the conserved oligomeric Golgi (COG) complex which is composed of eight different subunits and is required for normal Golgi morphology and localization. COG4 is required for normal Golgi function.
    • Cellular localization
      Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side.
    • Database links
    • Alternative names
      • CDG2J antibody
      • COD1 antibody
      • COG 4 antibody
      • COG complex subunit 4 antibody
      • Complexed with Dor1p antibody
      • Component of oligomeric golgi complex 4 antibody
      • Conserved oligomeric Golgi complex protein 4 antibody
      • Conserved oligomeric Golgi complex subunit 4 antibody
      • DKFZp586E1519 antibody
      see all

    Images

    • All lanes : Anti-COG4 antibody [EPR10111] (ab154795) at 1/1000 dilution

      Lane 1 : Hep G2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 89 kDa

    • ab154795 (1/500) staining COG4 in asynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized in 0.05% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.

      See Abreview

    • Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling COG4 with ab154795 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling COG4 with ab154795 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin embedded Human normal tonsil tissue using ab154795 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab154795 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human pancreas tissue using ab154795 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab154795 showing +ve staining.

    References

    ab154795 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.05% Triton X100 in PBS
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Feb 18 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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