Recombinant
RabMAb

Anti-COG7 antibody [EPR9942] (ab168362)

Overview

  • Product name
    Anti-COG7 antibody [EPR9942]
    See all COG7 primary antibodies
  • Description
    Rabbit monoclonal [EPR9942] to COG7
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human COG7 (UniProt: P83436).

  • Positive control
    • MOLT4, HeLa, MCF7, C6, RAW 264.7, PC12, and NIH 3T3 cell lysates; Human brain and kidney tissues; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR9942
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab168362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 86 kDa.
ICC/IF 1/100 - 1/250.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Required for normal Golgi function.
    • Involvement in disease
      Defects in COG7 are the cause of congenital disorder of glycosylation type 2E (CDG2E) [MIM:608779]. CDGs are a family of severe inherited diseases caused by a defect in protein N-glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions.
    • Sequence similarities
      Belongs to the COG7 family.
    • Cellular localization
      Golgi apparatus membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CDG2E antibody
      • COG complex subunit 7 antibody
      • cog7 antibody
      • COG7_HUMAN antibody
      • Component of oligomeric Golgi complex 7 antibody
      • Conserved oligomeric Golgi complex component 7 antibody
      • Conserved oligomeric Golgi complex subunit 7 antibody
      see all

    Images

    • All lanes : Anti-COG7 antibody [EPR9942] (ab168362) at 1/1000 dilution

      Lane 1 : MOlT4 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : C6 cell lysate
      Lane 5 : RAW 264.7 cell lysate
      Lane 6 : PC12 cell lysate
      Lane 7 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 86 kDa
    • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling COG7 with ab168362 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling COG7 with ab168362 at 1/100 dilution.
    • Immunofluorescent analysis of HeLa cells labeling COG7 with ab168362 at 1/100 dilution. DAPI nuclear staining (blue).
    • Flow cytometric analysis of permeabilized HeLa cells labeling COG7 (red) with ab168362 at 1/10 dilution, compared to a nonspecific control antibody (green).

    References

    ab168362 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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