Overview

  • Product nameAnti-Coilin antibody [Pdelta]
    See all Coilin primary antibodies
  • Description
    Mouse monoclonal [Pdelta] to Coilin
  • SpecificityTwo customers have reported no reactivity with mouse samples (either no reactivity or high non-specific background).
  • Tested applicationsSuitable for: ICC, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, African Green Monkey
  • Immunogen

    Recombinant fragment (C-terminus of Human coilin, 389 amino acids).

  • EpitopeMonoclonal Anti-coilin reacts with an epitope located between amino acids 363 and 481 of human coilin.
  • Positive control
    • HeLa nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab11822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionIs a component of the nuclear coiled bodies (CBS) which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBS disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin.
  • Tissue specificityFound in all the cell types examined.
  • Sequence similaritiesBelongs to the coilin family.
  • Cellular localizationNucleus. Nuclear coiled body located in the interchromatin space between the nucleolus and the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CLN 80 antibody
    • CLN80 antibody
    • COIL antibody
    • COIL_HUMAN antibody
    • Coilin antibody
    • Coilin p80 antibody
    • p80 antibody
    • P80 coilin antibody
    see all

Anti-Coilin antibody [Pdelta] images

  • ICC/IF image of ab11822 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11822, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Coilin antibody [Pdelta] (ab11822)

This product has been referenced in:
  • Carmona-Mora P  et al. The nuclear localization pattern and interaction partners of GTF2IRD1 demonstrate a role in chromatin regulation. Hum Genet N/A:N/A (2015). ICC/IF . Read more (PubMed: 26275350) »
  • Wang Y  et al. p32 is a novel target for viral protein ICP34.5 of herpes simplex virus type 1 and facilitates viral nuclear egress. J Biol Chem 289:35795-805 (2014). Read more (PubMed: 25355318) »

See all 16 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (H1299)
Specification H1299
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 12 2010

Thank you for your enquiry. We would like to confirm that this antibody has been tested and characterized for Western blot and ICC application. It recognizes human Coilin but it does not cross-react with Mouse However we would like to emphasize ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"