Overview

  • Product nameAnti-COL11A1 antibody
    See all COL11A1 primary antibodies
  • Description
    Rabbit polyclonal to COL11A1
  • Tested applicationsSuitable for: IHC-Fr, ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide from an internal sequence of Human COL11A1.

  • Positive control
    • K562 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/50. (see Abreview)
WB 1/500 - 1/1000. Detects a band of approximately >170 kDa (predicted molecular weight: 181 kDa).
ELISA 1/10000.

Target

  • FunctionMay play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils.
  • Tissue specificityCartilage, placenta and some tumor or virally transformed cell lines. Isoforms using exon IIA or IIB are found in the cartilage while isoforms using only exon IIB are found in the tendon.
  • Involvement in diseaseDefects in COL11A1 are the cause of Stickler syndrome type 2 (STL2) [MIM:604841]; also known as Stickler syndrome vitreous type 2. STL2 is an autosomal dominant form of Stickler syndrome, an inherited disorder that associates ocular signs with more or less complete forms of Pierre Robin sequence, bone disorders and sensorineural deafness. Ocular disorders may include juvenile cataract, myopia, strabismus, vitreoretinal or chorioretinal degeneration, retinal detachment, and chronic uveitis. Robin sequence includes an opening in the roof of the mouth (a cleft palate), a large tongue (macroglossia), and a small lower jaw (micrognathia). Bones are affected by slight platyspondylisis and large, often defective epiphyses. Juvenile joint laxity is followed by early signs of arthrosis. The degree of hearing loss varies among affected individuals and may become more severe over time. Syndrome expressivity is variable.
    Defects in COL11A1 are the cause of Marshall syndrome (MARSHS) [MIM:154780]. It is an autosomal dominant disorder with ocular, orofacial, auditory and skeletal manifestations. It shares several features with Stickler syndrome, such as midfacial hypoplasia, high myopia, and sensorineural-hearing deficit.
  • Sequence similaritiesBelongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 TSP N-terminal (TSPN) domain.
  • Post-translational
    modifications
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • COBA1_HUMAN antibody
    • COL11A1 antibody
    • COLL6 antibody
    • Collagen alpha 1 antibody
    • Collagen alpha-1(XI) chain antibody
    • collagen XI alpha 1 antibody
    • collagen XI, alpha 1 polypeptide antibody
    • collagen, type XI, alpha 1 antibody
    • STL2 antibody
    • STL3 antibody
    • XI chain precursor antibody
    see all

Anti-COL11A1 antibody images

  • All lanes : Anti-COL11A1 antibody (ab64883) at 1/500 dilution

    Lane 1 : extracts from K562 cells (5-30µg total protein)
    Lane 2 : extracts from K562 cells (5-30µg total protein) and 5-10µg of the immunising peptide.


    Predicted band size : 181 kDa
    Observed band size : 181 kDa
  • ab64883 staining of frozen human fetal heart tissue sections (IHC-Fr). Sections were fixed with paraformaldehyde and permeabilized by saponin. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour 30 minutes at 37°C. An Alexa Fluor conjugated goat anti-rabbit antibody was used as the secondary.

    This image is courtesy of an anonymous Abreview (April 2009)

  • Immunohistochemical analysis of frozen murine ovary tissue taken from post-natal day 23-29 wild-type (+/+) and ERß-null (-/-) mice.

    COL11A1 was stained with ab64883, at 1/200 dilution.

References for Anti-COL11A1 antibody (ab64883)

This product has been referenced in:
  • Lichtenberger BM  et al. Epidermal ß-catenin activation remodels the dermis via paracrine signalling to distinct fibroblast lineages. Nat Commun 7:10537 (2016). IHC ; Mouse . Read more (PubMed: 26837596) »
  • Akawi NA  et al. A response to Dr. Alzahrani's letter to the editor regarding the mechanism underlying fibrochondrogenesis. Gene 528:367-8 (2013). Read more (PubMed: 23906683) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lymph node)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer ph=6
Permeabilization No
Specification Lymph node
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative Paraformaldehyde
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Verified customer

Submitted Jul 11 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (MEF, R1 ES cells, E14 ES cells)
Specification MEF, R1 ES cells, E14 ES cells
Fixative Paraformaldehyde
Permeabilization Yes - Triton
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Submitted Jun 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (human fetal heart)
Specification human fetal heart
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% saponin in PBS
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 37°C
Username

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Verified customer

Submitted Apr 06 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"