The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/50. (see Abreview)
1/500 - 1/1000. Detects a band of approximately >170 kDa (predicted molecular weight: 181 kDa).
May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils.
Cartilage, placenta and some tumor or virally transformed cell lines. Isoforms using exon IIA or IIB are found in the cartilage while isoforms using only exon IIB are found in the tendon.
Involvement in disease
Defects in COL11A1 are the cause of Stickler syndrome type 2 (STL2) [MIM:604841]; also known as Stickler syndrome vitreous type 2. STL2 is an autosomal dominant form of Stickler syndrome, an inherited disorder that associates ocular signs with more or less complete forms of Pierre Robin sequence, bone disorders and sensorineural deafness. Ocular disorders may include juvenile cataract, myopia, strabismus, vitreoretinal or chorioretinal degeneration, retinal detachment, and chronic uveitis. Robin sequence includes an opening in the roof of the mouth (a cleft palate), a large tongue (macroglossia), and a small lower jaw (micrognathia). Bones are affected by slight platyspondylisis and large, often defective epiphyses. Juvenile joint laxity is followed by early signs of arthrosis. The degree of hearing loss varies among affected individuals and may become more severe over time. Syndrome expressivity is variable. Defects in COL11A1 are the cause of Marshall syndrome (MARSHS) [MIM:154780]. It is an autosomal dominant disorder with ocular, orofacial, auditory and skeletal manifestations. It shares several features with Stickler syndrome, such as midfacial hypoplasia, high myopia, and sensorineural-hearing deficit.
Belongs to the fibrillar collagen family. Contains 1 fibrillar collagen NC1 domain. Contains 1 TSP N-terminal (TSPN) domain.
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Secreted > extracellular space > extracellular matrix.
ab64883 staining of frozen human fetal heart tissue sections (IHC-Fr). Sections were fixed with paraformaldehyde and permeabilized by saponin. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour 30 minutes at 37°C. An Alexa Fluor conjugated goat anti-rabbit antibody was used as the secondary.
This image is courtesy of an anonymous Abreview (April 2009)
Immunohistochemistry (Frozen sections) - Anti-COL11A1 antibody (ab64883)Image from Zalewski A et al., PLoS One. 2012;7(1):e29937. Epub 2012 Jan 10.Fig 1.; doi:10.1371/journal.pone.0029937; January 10, 2012, PLoS ONE 7(1): e29937.
Immunohistochemical analysis of frozen murine ovary tissue taken from post-natal day 23-29 wild-type (+/+) and ERß-null (-/-) mice.
COL11A1 was stained with ab64883, at 1/200 dilution.