Overview

  • Product name
  • Description
    Rabbit polyclonal to COL12A1
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 768-892 of Human COL12A1.

  • Positive control
    • Human skeletal muscle tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 333 kDa.

Target

  • Relevance
    Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • BA209D8.1 antibody
    • COCA1 antibody
    • COL12A1 antibody
    • COL12A1L antibody
    • Collagen alpha-1(XII) chain antibody
    • collagen type XII proteoglycan antibody
    • collagen, type XII, alpha 1 antibody
    • DJ234P15.1 antibody
    see all

Images

  • Lane 1 :
    Lane 2 :

    Lane 1 : Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
    Lane 2 : Human cell line U-2 OS


    Predicted band size : 333 kDa
  • ab121304 staining COL12A1 in Paraffin-embedded Human skeletal muscle tissue by Immunohistochemistry.

References

ab121304 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab121304.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up