Anti-COL19A1 antibody (ab122793)
Key features and details
- Rabbit polyclonal to COL19A1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-COL19A1 antibody -
Description
Rabbit polyclonal to COL19A1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: VTVRDKTEES CPILRIEGHQ LTYDNINKLE VSGFDLGDSF SLRRAFCESD KTCFKLGSAL LIRDTIKIFP KGLPEEYSVA AMFRVRRNAK KERWFLWQVL, corresponding to amino acids of Human COL19A1.
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Positive control
- Human urinary bladder tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May act as a cross-bridge between fibrils and other extracellular matrix molecules. Involved in skeletal myogenesis in the developing esophagus. May play a role in organization of the pericellular matrix or the sphinteric smooth muscle. -
Tissue specificity
Localized to vascular, neuronal, mesenchymal, and some epithelial basement membrane zones in umbilical cord. -
Sequence similarities
Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.
Contains 1 TSP N-terminal (TSPN) domain. -
Domain
The numerous interruptions in the triple helix may make this molecule either elastic or flexible. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 1310 Human
- Omim: 120165 Human
- SwissProt: Q14993 Human
- Unigene: 444842 Human
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Alternative names
- COJA1_HUMAN antibody
- COL19A1 antibody
- COL9A1L antibody
see all
Images
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Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm and cell junctions. Recommended concentration of ab122793 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab122793, at 1/200 dilution, staining COL19A1 in paraffin-embedded Human urinary bladder tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab122793 has not yet been referenced specifically in any publications.