The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionMay act as a cross-bridge between fibrils and other extracellular matrix molecules. Involved in skeletal myogenesis in the developing esophagus. May play a role in organization of the pericellular matrix or the sphinteric smooth muscle.
Tissue specificityLocalized to vascular, neuronal, mesenchymal, and some epithelial basement membrane zones in umbilical cord.
Sequence similaritiesBelongs to the fibril-associated collagens with interrupted helices (FACIT) family. Contains 1 TSP N-terminal (TSPN) domain.
DomainThe numerous interruptions in the triple helix may make this molecule either elastic or flexible.
Post-translational modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Cellular localizationSecreted > extracellular space > extracellular matrix.