Overview

  • Product nameAnti-COL1A2 antibody
    See all COL1A2 primary antibodies
  • Description
    Rabbit polyclonal to COL1A2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Dog, Monkey
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1057 and 1339 of COL1A2 (NP_000080).

  • Positive control
    • WB: 293T and A431 cell lysate ICC/IF: A431 cell

Properties

Applications

Our Abpromise guarantee covers the use of ab96723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 129 kDa.
ICC/IF 1/100 - 1/200.

Target

  • FunctionType I collagen is a member of group I collagen (fibrillar forming collagen).
  • Tissue specificityForms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • Involvement in diseaseEhlers-Danlos syndrome 7B
    Osteogenesis imperfecta 1
    Osteogenesis imperfecta 2
    Ehlers-Danlos syndrome, autosomal recessive, cardiac valvular form
    Osteogenesis imperfecta 3
    Osteogenesis imperfecta 4
    A chromosomal aberration involving COL1A2 may be a cause of lipoblastomas, which are benign tumors resulting from transformation of adipocytes, usually diagnosed in children. Translocation t(7;8)(p22;q13) with PLAG1.
  • Sequence similaritiesBelongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
  • DomainThe C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
  • Post-translational
    modifications
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha 2 collagen type I antibody
    • Alpha 2 type I collagen antibody
    • Alpha 2 type I procollagen antibody
    • Alpha 2(I) collagen antibody
    • Alpha 2(I) procollagen antibody
    • Alpha-2 type I collagen antibody
    • CO1A2_HUMAN antibody
    • COL1A2 antibody
    • Collagen alpha 2(I) chain antibody
    • Collagen alpha-2(I) chain antibody
    • Collagen I alpha 2 polypeptide antibody
    • Collagen of skin tendon and bone alpha 2 chain antibody
    • Collagen type I alpha 2 antibody
    • OI4 antibody
    • Osteogenesis imperfecta type IV antibody
    • Type I procollagen antibody
    see all

Anti-COL1A2 antibody images

  • All lanes : Anti-COL1A2 antibody (ab96723) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 129 kDa
  • Immunofluorescence analysis of COL1A2 in paraformaldehyde fixed A431 cell, using ab96723 at a 1/200 dilution.
    Lower image: merged with DNA probe.

References for Anti-COL1A2 antibody (ab96723)

This product has been referenced in:
  • Zimmerman KA  et al. Calreticulin regulates transforming growth factor-ß-stimulated extracellular matrix production. J Biol Chem 288:14584-98 (2013). Read more (PubMed: 23564462) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Bos taurus Tissue lysate - other (Follicular fluids from ovarian follicles)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 35 µg
Specification Follicular fluids from ovarian follicles
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 18 2015

Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Rat Tissue lysate - whole (prostate)
Specification prostate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Xu Cheng

Verified customer

Submitted Sep 08 2014

Application Western blot
Sample Mouse Cell lysate - whole cell (Primary calvarial cells)
Loading amount 20 µg
Specification Primary calvarial cells
Gel Running Conditions Reduced Denaturing (10%)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Erica Perryn

Verified customer

Submitted Dec 20 2012

DISCOUNT CODE: ***
Expiration date: January 18th, 2013

I am very pleased to hear you would like to accept our offer and test ab96723 in rat. This code will give you: 1 free primary antibody before the expiration date. To redeem this off...

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Thank you for contacting us.

Yes, that is no problem and we are looking forward to receiving your Abreview. I am sending you another discount code for testing ab96723 in mouse, but please ensure that you use it before it expires, as extendin...

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Thank you for your patience. Yes, the lab confirmed that denaturing conditions have been used: The proteins were extracted in a RIPA buffer containing SDS. I hope this information is helpful to you. Please do not hesitate to contact us if you n...

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Thank you for your patience. I received the following information from the lab: The samples were boiled (at 100 degree C for 10 mins) under reducing condition. I assume this means also under denaturing conditions, but I am checking with them to con...

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