Overview

  • Product nameAnti-COL4A3BP antibody
    See all COL4A3BP primary antibodies
  • Description
    Rabbit polyclonal to COL4A3BP
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Chinese Hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide from a region between residue 1 and 50 of human COL4A3BP (NP_005704.1)

  • Positive control
    • Whole cell lysate from Hela and 293T cells.

Associated products

Applications

Our Abpromise guarantee covers the use of ab72534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use at 1-4µg/mg of lysate.
    WB: 1/2500 - 1/10000. Detects bands of approximately 70, 71, 110 and 230 kDa (predicted molecular weight: 71 and 70 kDa for isoforms 1 and 2 respectively).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay mediate the intracellular trafficking of ceramide in a non-vesicular manner.
    • Tissue specificityWidely expressed.
    • Sequence similaritiesContains 1 PH domain.
      Contains 1 START domain.
    • DomainThe START domain recognizes ceramide and mediates the intermembrane transfer of ceramide.
      The PH domain targets the Golgi apparatus.
      The FFAT motif is required for interaction with VAPA and VAPB.
    • Post-translational
      modifications
      Phosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity.
    • Cellular localizationCytoplasm. Golgi apparatus. Endoplasmic reticulum. Preferentially localized to the Golgi apparatus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha 3 type IV collagen binding protein antibody
      • C43BP_HUMAN antibody
      • Ceramide transfer protein antibody
      • Ceramide transporter antibody
      • CERT antibody
      • Cert protein antibody
      • CERTL antibody
      • col4a3bp antibody
      • Collagen type IV alpha 3 (Goodpasture antigen) binding protein antibody
      • Collagen type IV alpha 3 binding protein antibody
      • Collagen type IV alpha-3-binding protein antibody
      • FLJ20597 antibody
      • Goodpasture antigen binding protein antibody
      • Goodpasture antigen-binding protein antibody
      • GPBP antibody
      • hCERT antibody
      • Lipid transfer protein CERTL antibody
      • Procollagen type IV alpha 3 (Goodpasture antigen) binding protein antibody
      • StAR related lipid transfer (START) domain containing 11 antibody
      • StAR related lipid transfer protein 11 antibody
      • StAR-related lipid transfer protein 11 antibody
      • StARD 11 antibody
      • StARD11 antibody
      • START domain containing 11 antibody
      • START domain containing protein 11 antibody
      • START domain-containing protein 11 antibody
      see all

    Anti-COL4A3BP antibody images

    • All lanes : Anti-COL4A3BP antibody (ab72534) at 0.1 µg/ml

      Lane 1 : Whole cell lysate from Hela cells at 50 µg
      Lane 2 : Whole cell lysate from Hela cells at 15 µg
      Lane 3 : Whole cell lysate from Hela cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg


      Predicted band size : 71 kDa
      Observed band size : 70,71 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 110 kDa,230 kDa. We are unsure as to the identity of these extra bands.
    • Immunoprecipitation/ Western Blot of COL4A3BP.
      Lane 1: ab72534 at 3µg/mg whole cell lysate.
      Lane 2: Control IgG.
      ab72534 at 1µg/ml for WB.
      Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
      Chemiluminescence with an exposure time of 30 seconds.

    References for Anti-COL4A3BP antibody (ab72534)

    ab72534 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab72534.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"