The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at 1-4µg/mg of lysate.
WB: 1/2500 - 1/10000. Detects bands of approximately 70, 71, 110 and 230 kDa (predicted molecular weight: 71 and 70 kDa for isoforms 1 and 2 respectively).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay mediate the intracellular trafficking of ceramide in a non-vesicular manner.
DomainThe START domain recognizes ceramide and mediates the intermembrane transfer of ceramide. The PH domain targets the Golgi apparatus. The FFAT motif is required for interaction with VAPA and VAPB.
Post-translational modificationsPhosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity.
Cellular localizationCytoplasm. Golgi apparatus. Endoplasmic reticulum. Preferentially localized to the Golgi apparatus.
Collagen type IV alpha 3 (Goodpasture antigen) binding protein antibody
Collagen type IV alpha 3 binding protein antibody
Collagen type IV alpha-3-binding protein antibody
Goodpasture antigen binding protein antibody
Goodpasture antigen-binding protein antibody
Lipid transfer protein CERTL antibody
Procollagen type IV alpha 3 (Goodpasture antigen) binding protein antibody
StAR related lipid transfer (START) domain containing 11 antibody
StAR related lipid transfer protein 11 antibody
StAR-related lipid transfer protein 11 antibody
StARD 11 antibody
START domain containing 11 antibody
START domain containing protein 11 antibody
START domain-containing protein 11 antibody
Anti-COL4A3BP antibody images
Western blot - COL4A3BP antibody (ab72534)
All lanes : Anti-COL4A3BP antibody (ab72534) at 0.1 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg Lane 2 : Whole cell lysate from Hela cells at 15 µg Lane 3 : Whole cell lysate from Hela cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg
Immunoprecipitation/ Western Blot of COL4A3BP.
Lane 1: ab72534 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72534 at 1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 30 seconds.
References for Anti-COL4A3BP antibody (ab72534)
has not yet been referenced specifically in any publications.
Publishing research using ab72534? Please let us know so that we can cite the reference in this datasheet.
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