Overview

  • Product nameAnti-COL4A3BP antibody
    See all COL4A3BP primary antibodies
  • Description
    Rabbit polyclonal to COL4A3BP
  • Tested applicationsSuitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to a region between residues 300 and 350 of human COL4A3BP (NP_005704.1)

  • Positive control
    • In Western Blot, this antibody gave a positive signal in HeLa and 293T whole cell lysates. This antibody gave a positive result when used in the following formaldehyde/methanol fixed cell lines: DU145.

Associated products

Applications

Our Abpromise guarantee covers the use of ab72536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ICC/IF
  • Application notesIP: Use at 1 - 4µg/mg of lysate.
    WB: 1/2500 - 1/10000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay mediate the intracellular trafficking of ceramide in a non-vesicular manner.
    • Tissue specificityWidely expressed.
    • Sequence similaritiesContains 1 PH domain.
      Contains 1 START domain.
    • DomainThe START domain recognizes ceramide and mediates the intermembrane transfer of ceramide.
      The PH domain targets the Golgi apparatus.
      The FFAT motif is required for interaction with VAPA and VAPB.
    • Post-translational
      modifications
      Phosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity.
    • Cellular localizationCytoplasm. Golgi apparatus. Endoplasmic reticulum. Preferentially localized to the Golgi apparatus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha 3 type IV collagen binding protein antibody
      • C43BP_HUMAN antibody
      • Ceramide transfer protein antibody
      • Ceramide transporter antibody
      • CERT antibody
      • Cert protein antibody
      • CERTL antibody
      • col4a3bp antibody
      • Collagen type IV alpha 3 (Goodpasture antigen) binding protein antibody
      • Collagen type IV alpha 3 binding protein antibody
      • Collagen type IV alpha-3-binding protein antibody
      • FLJ20597 antibody
      • Goodpasture antigen binding protein antibody
      • Goodpasture antigen-binding protein antibody
      • GPBP antibody
      • hCERT antibody
      • Lipid transfer protein CERTL antibody
      • Procollagen type IV alpha 3 (Goodpasture antigen) binding protein antibody
      • StAR related lipid transfer (START) domain containing 11 antibody
      • StAR related lipid transfer protein 11 antibody
      • StAR-related lipid transfer protein 11 antibody
      • StARD 11 antibody
      • StARD11 antibody
      • START domain containing 11 antibody
      • START domain containing protein 11 antibody
      • START domain-containing protein 11 antibody
      see all

    Anti-COL4A3BP antibody images

    • ICC/IF image of ab72536 stained DU145. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab72536 at (5 µg/mL) overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti Rabbit  (96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • All lanes : Anti-COL4A3BP antibody (ab72536) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 71 kDa
      Observed band size : 71 kDa
      Additional bands at : 100 kDa,28 kDa,60 kDa,70 kDa (possible isoform). We are unsure as to the identity of these extra bands.
    • Detection of Human COL4A3BP by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72536 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

    References for Anti-COL4A3BP antibody (ab72536)

    This product has been referenced in:
    • Burgett AW  et al. Natural products reveal cancer cell dependence on oxysterol-binding proteins. Nat Chem Biol 7:639-47 (2011). WB ; Human . Read more (PubMed: 21822274) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"