Overview

  • Product nameAnti-COL4A3BP antibody
    See all COL4A3BP primary antibodies
  • Description
    Goat polyclonal to COL4A3BP
  • Specificityab77186 is expected to recognise both reported isoforms (NP_005704.1 and NP_112729.1).
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Horse, Chicken, Cow, Dog, Human, Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide:

    DHDSAPLNNRC

    , from the internal region of Human COL4A3BP protein sequence (NP_005704.1; NP_112729.1).

  • Positive control
    • Mouse heart lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab77186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesPeptide ELISA: Antibody detection limit dilution 1/32000.
    WB: Use at a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 71 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay mediate the intracellular trafficking of ceramide in a non-vesicular manner.
    • Tissue specificityWidely expressed.
    • Sequence similaritiesContains 1 PH domain.
      Contains 1 START domain.
    • DomainThe START domain recognizes ceramide and mediates the intermembrane transfer of ceramide.
      The PH domain targets the Golgi apparatus.
      The FFAT motif is required for interaction with VAPA and VAPB.
    • Post-translational
      modifications
      Phosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity.
    • Cellular localizationCytoplasm. Golgi apparatus. Endoplasmic reticulum. Preferentially localized to the Golgi apparatus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha 3 type IV collagen binding protein antibody
      • C43BP_HUMAN antibody
      • Ceramide transfer protein antibody
      • Ceramide transporter antibody
      • CERT antibody
      • Cert protein antibody
      • CERTL antibody
      • col4a3bp antibody
      • Collagen type IV alpha 3 (Goodpasture antigen) binding protein antibody
      • Collagen type IV alpha 3 binding protein antibody
      • Collagen type IV alpha-3-binding protein antibody
      • FLJ20597 antibody
      • Goodpasture antigen binding protein antibody
      • Goodpasture antigen-binding protein antibody
      • GPBP antibody
      • hCERT antibody
      • Lipid transfer protein CERTL antibody
      • Procollagen type IV alpha 3 (Goodpasture antigen) binding protein antibody
      • StAR related lipid transfer (START) domain containing 11 antibody
      • StAR related lipid transfer protein 11 antibody
      • StAR-related lipid transfer protein 11 antibody
      • StARD 11 antibody
      • StARD11 antibody
      • START domain containing 11 antibody
      • START domain containing protein 11 antibody
      • START domain-containing protein 11 antibody
      see all

    Anti-COL4A3BP antibody images

    • Anti-COL4A3BP antibody (ab77186) at 0.5 µg/ml + mouse heart lysate in RIPA buffer at 35 µg

      Predicted band size : 71 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-COL4A3BP antibody (ab77186)

    ab77186 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us with your question about ab77186.
    Ab77186 is predicted to work with chicken samples based on sequence homology, though we don't know for sure until it is tested. It is quite possible that the antibody will work in tissu...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"