Anti-CERT antibody (ab151285)
Key features and details
- Rabbit polyclonal to CERT
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CERT antibody
See all CERT primary antibodies -
Description
Rabbit polyclonal to CERT -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Xenopus tropicalis -
Immunogen
Synthetic peptide corresponding to Human CERT aa 1-100 (N terminal).
Database link: Q9Y5P4-1 -
Positive control
- A431, HeLa, HepG2 and H1299 whole cell lysates; methanol-fixed A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 68 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 68 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
May mediate the intracellular trafficking of ceramide in a non-vesicular manner. -
Tissue specificity
Widely expressed. -
Sequence similarities
Contains 1 PH domain.
Contains 1 START domain. -
Domain
The START domain recognizes ceramide and mediates the intermembrane transfer of ceramide.
The PH domain targets the Golgi apparatus.
The FFAT motif is required for interaction with VAPA and VAPB. -
Post-translational
modificationsPhosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity. -
Cellular localization
Cytoplasm. Golgi apparatus. Endoplasmic reticulum. Preferentially localized to the Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 286797 Cow
- Entrez Gene: 10087 Human
- Entrez Gene: 68018 Mouse
- Entrez Gene: 365652 Rat
- Entrez Gene: 432007 Xenopus laevis
- Omim: 604677 Human
- SwissProt: Q9GKI7 Cow
- SwissProt: Q9Y5P4 Human
see all -
Alternative names
- Alpha 3 type IV collagen binding protein antibody
- C43BP_HUMAN antibody
- Ceramide transfer protein antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab151285 has not yet been referenced specifically in any publications.