Our Abpromise guarantee covers the use of ab112110 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 67 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
Immunohistochemcial analysis of PFA-fixed frozen murine muscular and connective tissue sections, labelling COL8A2 with ab112110 at a dilution of 1/100 incubated for 72 hours at 25°C in 1% BSA & 3% donkey serum in PBST. Permeabilization was with PSBT. Blocking was with 3% donkey serum & 1% BSA in PBST incubated for 1 hour at 25°C. The secondary used was a donkey ant-rabbit polyclonal Alexa Fluor® 488 at 1/1000. Positive staing was found to be present in the extracellular space at the edge of the muscle tissue. A red channel was included to control for tissue autofluorescence. Within additional sections (not shown) negative controls, where the primary antibody ab112110 was not present showed no staining.
ab112110 has not yet been referenced specifically in any publications.