• Product nameAnti-COL8A2 antibody
    See all COL8A2 primary antibodies
  • Description
    Rabbit polyclonal to COL8A2
  • Tested applicationsSuitable for: IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 285-334 (AAGLPGPQGP SGAKGEPGTR GPPGLIGPTG YGMPGLPGPK GDRGPAGVPG) of Human COL8A2 (NP_005193).

  • Positive control
    • Human fetal liver lysate.



Our Abpromise guarantee covers the use of ab112110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100.
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 67 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • RelevanceCollagen VIII is a member of the 'short chain collagen' subfamily, and is a major component of the hexagonal lattice in the Descemet membrane. Type VIII collagen molecules, expressed by corneal and vascular endothelial cells, are heterotrimers composed of 2 genetically distinct polypeptides, alpha-1(VIII) (COL8A1) and alpha-2(VIII) (COL8A2).
  • Cellular localizationSecreted; extracellular space; extracellular matrix; basement membrane.
  • Database links
  • Alternative names
    • AI429819 antibody
    • Collagen, type VIII, alpha 2 antibody
    • FECD antibody
    • FECD1 antibody
    • FLJ00201 antibody
    • MGC116970 antibody
    • MGC116972 antibody
    • MGC90765 antibody
    • PPCD antibody
    • PPCD2 antibody
    • RP23-113P22.2 antibody
    • RP4-665N4.3 antibody
    see all

Anti-COL8A2 antibody images

  • Immunohistochemcial analysis of PFA-fixed frozen murine muscular and connective tissue sections,  labelling COL8A2 with ab112110 at a dilution of 1/100 incubated for 72 hours at 25°C in 1% BSA & 3% donkey serum in PBST. Permeabilization was with PSBT. Blocking was with 3% donkey serum & 1% BSA in PBST incubated for 1 hour at 25°C.  The secondary used was a donkey ant-rabbit polyclonal Alexa Fluor® 488 at 1/1000. Positive staing was found to be present in the extracellular space at the edge of the muscle tissue. A red channel was included to control for tissue autofluorescence. Within additional sections (not shown) negative controls, where the primary antibody ab112110 was not present showed no staining.

    See Abreview

  • Anti-COL8A2 antibody (ab112110) at 1 µg/ml + Human Fetal Liver lysate at 10 µg

    Predicted band size : 67 kDa

References for Anti-COL8A2 antibody (ab112110)

ab112110 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Adult connective/muscle tissue)
Permeabilization Yes - PBST (PBS / 0.1% v/v Triton X-100 (Sigma))
Specification Adult connective/muscle tissue
Blocking step 3% donkey serum / 1% BSA (Life Technologies) in PBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Paraformaldehyde

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Submitted Sep 15 2015