Overview

  • Product name
    Anti-COLEC11 antibody
  • Description
    Rabbit polyclonal to COLEC11
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide selected from the N-terminal region of Human COLEC11 conjugated to KLH (NP_076932).

  • Positive control
    • MDA-MB231 cell line lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    ab91483 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/50 - 1/100. Predicted molecular weight: 29 kDa.
ELISA 1/1000.

Target

  • Function
    Lectin that binds to various sugars including fucose and mannose. Has a higher affinity for fucose compared to mannose. Does not bind to glucose, N-acetylglucosamine and N-acetylgalactosamine. Also binds lipopolysaccharides (LPS). Involved in fundamental development serving as a guidance cue for neural crest cell migration.
  • Tissue specificity
    Ubiquitous.
  • Involvement in disease
    Defects in COLEC11 are the cause of 3MC syndrome type 2 (3MC2) [MIM:265050]. 3MC2 is an a disorder characterized by facial dysmorphism that includes hypertelorism, blepharophimosis, blepharoptosis and highly archedDE eyebrows, cleft lip and/or palate, craniosynostosis, learning disability and genital, limb and vesicorenal anomalies. The term 3MC syndrome includes Carnevale, Mingarelli, Malpuech, and Michels syndromes.
  • Sequence similarities
    Belongs to the COLEC10/COLEC11 family.
    Contains 1 C-type lectin domain.
    Contains 1 collagen-like domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CL K1 antibody
    • CL K1 I antibody
    • CL K1 II antibody
    • CL K1 IIa antibody
    • CL K1 IIb antibody
    • CL-K1 antibody
    • CLK1 antibody
    • COL11_HUMAN antibody
    • COLEC 11 antibody
    • COLEC11 antibody
    • Collectin 11 antibody
    • Collectin kidney I antibody
    • Collectin kidney protein 1 antibody
    • Collectin sub family member 11 antibody
    • Collectin-11 antibody
    • Collectin11 antibody
    • DKFZp686N1868 antibody
    • MGC129470 antibody
    • MGC129471 antibody
    • MGC3279 antibody
    see all

Images

  • Anti-COLEC11 antibody (ab91483) at 1/50 dilution + MDA-MB231 cell line lysate at 35 µg

    Predicted band size : 29 kDa
  • ICC/IF image of ab91483 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91483, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab91483 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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