Anti-Collagen I + Collagen III antibody (ab24135)

Overview

  • Product nameAnti-Collagen I + Collagen III antibodySee all Collagen I + Collagen III primary antibodies ...
  • Description
    Rabbit polyclonal to Collagen I + Collagen III
  • SpecificitySpecificity is measured by ELISA using pure pig collagen types I and III. No binding to pig serum proteins is seen at similar dilutions of the antibody. No cross reactivity seen against pig basement membrane collagens type IV and V in immunostaining.
  • Tested applicationsDot Blot, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Pig
  • Immunogen

    Full length native collagen type I and III from pig skin.

  • Positive control
    • Pig kidney and skin. Cultured porcine cells.

Properties

  • FormLyophilised:Reconstitute with 1ml of distilled water and add preservative if required.
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: Whole serum
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPooled antisera are passed over DEAE-cellulose to produce an IgG enriched fraction. Antibodies specific to type I and type III pig collagen are then immobilised by affinity chromatography and eluted with an acidic buffer. The final purification steps are neutralisation, dialysis and lyophilisation.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab24135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot Blot Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
IHC-Fr 1/10 - 1/20.

Target

  • RelevanceCollagen I is a fibrillar collagen found in most connective tissues, and the only component of the collagen found in cartilage. Mutations in this gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with Collagen I. Mutations in this gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
  • Cellular localizationSecreted
  • Alternative names
    • Alpha 1 type I collagen antibody
    • Alpha 2 type I collagen antibody
    • COL1A1 antibody
    • COL1A1 antibody
    • COL1A2 antibody
    • COL3A1 antibody
    • Collagen fetal antibody
    • Collagen type I alpha 1 antibody
    • Collagen type I alpha 2 antibody
    • Collagen type III alpha 1 antibody
    • EDS4A antibody
    • Ehlers Danlos syndrome type IV autosomal dominant antibody
    • OI4 antibody
    • Type I collagen alpha 1 chain antibody
    • Type I procollagen antibody
    see all

References for Anti-Collagen I + Collagen III antibody (ab24135)

ab24135 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

That antibody, ab24135, is unlikely to be a good choice, though it is raised against pig collagen. It will be difficult to find anti-collagen antibodies that are reactive with one species and not another, given t...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"