Anti-Collagen I + II + III + IV + V antibody (ab36064)


  • Product nameAnti-Collagen I + II + III + IV + V antibody
    See all Collagen I + II + III + IV + V primary antibodies
  • Description
    Rabbit polyclonal to Collagen I + II + III + IV + V
  • Tested applicationsSuitable for: ICC/IF, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native Collagen I + II + III + IV + V proteins (purified) from human placenta.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.15M Sodium chloride, 0.01M Sodium phosphate, pH 7.5, 0.1% mannitol, 0.1% dextran.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody is affinity isolated. It was purified by affinity chromatography on immobilized Collagen types I-V.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab36064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/40.
ELISA 1/2000 - 1/8000.
IHC-Fr 1/10 - 1/40.


  • FunctionType I collagen is a member of group I collagen (fibrillar forming collagen).
  • Tissue specificityForms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
  • Involvement in diseaseCaffey disease
    Ehlers-Danlos syndrome, classic type
    Ehlers-Danlos syndrome 7A
    Osteogenesis imperfecta 1
    Osteogenesis imperfecta 2
    Osteogenesis imperfecta 3
    Osteogenesis imperfecta 4
    A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
  • Sequence similaritiesBelongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 VWFC domain.
  • DomainThe C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
  • Post-translational
    Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.
    O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
  • Cellular localizationSecreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha-1 type I collagen antibody
    • CO1A1_HUMAN antibody
    • COL1A1 antibody
    • COL2A1 antibody
    • COL3A1 antibody
    • COL4A1 antibody
    • COL5A1 antibody
    • Collagen alpha 1 chain antibody
    • Collagen alpha 1(I) chain antibody
    • Collagen alpha 1(II) chain antibody
    • Collagen alpha 1(III) chain antibody
    • Collagen alpha 1(IV) chain antibody
    • Collagen alpha 1(V) chain antibody
    • Collagen alpha-1(I) chain antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen II alpha 1 polypeptide antibody
    • Collagen IV alpha 1 polypeptide antibody
    • Collagen type I alpha 1 antibody
    • Collagen type II alpha 1 antibody
    • Collagen type III alpha 1 antibody
    • Collagen type IV alpha 1 antibody
    • Collagen type V alpha 1 antibody
    • EDS4A antibody
    see all

References for Anti-Collagen I + II + III + IV + V antibody (ab36064)

ab36064 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Liver)
Loading amount 300 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (4-12% precast)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted Jun 15 2012

Thank you for your reply. Below is the discount for testing ab36064.

Please let me know if there is anything else I can help you with.

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Thank you for contacting Abcam.

The testing discount codes are normally issued before you purchase the antibody, this is so we can track it in our system. However in this case I will make an exception and issue the code post purchase. To do t...

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Thank you for contacting Abcam.
I have spoken to the lab about the epitopes that are recognized by ab36064 and the epitope has not been mapped for this antibody and because it is a polyclonal antibody, it will recognize multiple epitopes.
I b...

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