Anti-Collagen I + II + III + IV + V antibody (ab36064)

Overview

  • Product nameAnti-Collagen I + II + III + IV + V antibody
    See all Collagen I + II + III + IV + V primary antibodies
  • Description
    Rabbit polyclonal to Collagen I + II + III + IV + V
  • SpecificityThis antibody was found to cross react in ELISA with the following: Collagen types 3/4/5: 100%; Collagen types 1 and 2: 70%; Human fibronectin, albumin and immunoglobulins all <0.5%.
  • Tested applicationsICC/IF, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native Collagen I + II + III + IV + V proteins (purified) from human placenta.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.15M Sodium chloride, 0.01M Sodium phosphate, pH 7.5, 0.1% mannitol, 0.1% dextran.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody is affinity isolated. It was purified by affinity chromatography on immobilized Collagen types I-V.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab36064 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/40.
ELISA 1/2000 - 1/8000.
IHC-Fr 1/10 - 1/40.

Target

  • RelevanceCollagen I is a member of group I collagen (fibrillar forming collagen), it forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite. Collagen II is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. High expression of isoform 2 in juvenile chondrocyte and low in fetal chondrocyte. Collagen III occurs in most soft connective tissues along with collagen I. Collagen IV is the major structural component of glomerular basement membranes, forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Collagen V is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Collagen V binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
  • Cellular localizationExtracellular
  • Database links
  • Alternative names
    • COL11A3 antibody
    • COL1A1 antibody
    • COL2A1 antibody
    • COL3A1 antibody
    • COL4A1 antibody
    • COL5A1 antibody
    • Collagen alpha 1 chain antibody
    • Collagen alpha 1 II chain precursor contains chondrocalcin antibody
    • Collagen alpha 1 III chain precursor antibody
    • Collagen alpha 1 V chain precursor antibody
    • Collagen alpha 1(I) chain antibody
    • Collagen alpha 1(II) chain antibody
    • Collagen alpha 1(III) chain antibody
    • Collagen alpha 1(IV) chain antibody
    • Collagen alpha 1(V) chain antibody
    • Collagen alpha 2 I chain precursor antibody
    • Collagen alpha 2 V chain precursor antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen II alpha 1 polypeptide antibody
    • Collagen IV alpha 1 polypeptide antibody
    • Collagen type I alpha 1 antibody
    • Collagen type I alpha 2 antibody
    • Collagen type II alpha 1 antibody
    • Collagen type III alpha 1 antibody
    • Collagen type IV alpha 1 antibody
    • Collagen type V alpha 1 antibody
    • Collagen type V alpha 2 antibody
    • EDS4A antibody
    • Ehlers Danlos syndrome type IV autosomal dominant antibody
    • OI4 antibody
    • Primary osteoarthritis spondyloepiphyseal dysplasia congenital antibody
    • SEDC antibody
    see all

References for Anti-Collagen I + II + III + IV + V antibody (ab36064)

ab36064 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Liver)
Loading amount 300 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (4-12% precast)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 15 2012

Thank you for your reply. Below is the discount for testing ab36064.

Please let me know if there is anything else I can help you with.

DISCOUNT CODE: *********
Expiration date: ************

I am very pleased to hear ...

Read More

Thank you for contacting Abcam.

The testing discount codes are normally issued before you purchase the antibody, this is so we can track it in our system. However in this case I will make an exception and issue the code post purchase. To do t...

Read More

Thank you for contacting Abcam.
I have spoken to the lab about the epitopes that are recognized by ab36064 and the epitope has not been mapped for this antibody and because it is a polyclonal antibody, it will recognize multiple epitopes.
I b...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"