Anti-Collagen I + II + III antibody [1.BB.749] (ab61969)

Overview

  • Product nameAnti-Collagen I + II + III antibody [1.BB.749]See all Collagen I + II + III primary antibodies ...
  • Description
    Mouse monoclonal [1.BB.749] to Collagen I + II + III
  • SpecificityThis antibody reacts with native as well as with heat denatured collagen types I, II, III. It does not react with other collagen types (IV and V). Human plasma proteins do not interfere with binding to collagen.
  • Tested applicationsELISA, RIA, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human Collagen Type I, II, III.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityPurified IgM
  • Purification notesThis antibody was purified by PEG6000 precipitation and Sepharose 6B gel filtration.
  • Clonality Monoclonal
  • Clone number1.BB.749
  • IsotypeIgM
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab61969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
RIA
IHC-Fr
Immunohistochemistry (acetone fixed)
  • Application notesSandwich ELISA: 1/1000 to determine concentrations of interstitial collagens in human fluid.
    IHC-Fr: 1/20.
    RIA: Use at an assay dependent dilution.

    Is unsuitable for IHC-P.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceCollagen I is a fibrillar collagen found in most connective tissues, and the only component of the collagen found in cartilage. Mutations in this gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. Type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Collagen type II is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with Collagen I. Mutations in this gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
    • Database links
    • Alternative names
      • ALPHA 1 antibody
      • ALPHA 1 CHAIN antibody
      • Alpha 1 type II col antibody
      • ALPHA 2 antibody
      • ALPHA 2 CHAIN antibody
      • ANFH antibody
      • AOM antibody
      • Cartilage collagen antibody
      • Chondrocalcin antibody
      • COL 3A1 antibody
      • COL11A3 antibody
      • COL1A1 antibody
      • COL1A1 PDGFB FUSION GENE antibody
      • COL1A2 antibody
      • COL2A1 antibody
      • COL3A1 antibody
      • Collagen alpha 1(II) chain antibody
      • Collagen alpha 1(III) chain antibody
      • Collagen I alpha 1 polypeptide antibody
      • Collagen I alpha 2 polypeptide antibody
      • Collagen II alpha 1 polypeptide antibody
      • Collagen III alpha 1 chain precursor antibody
      • Collagen III alpha 1 polypeptide antibody
      • COLLAGEN OF CARTILAGE antibody
      • Collagen Of Skin Tendon And Bone antibody
      • Collagen Type 1 antibody
      • Collagen type II alpha 1 (primary osteoarthritis spondyloepiphyseal dysplasia congenital) antibody
      • COLLAGEN TYPE II antibody
      • EDS4A antibody
      • Fetal collagen antibody
      • MGC131516 antibody
      • OI EDS COMBINED SYNDROME antibody
      • OI4 antibody
      • Osteogenesis Imperfecta Type IV antibody
      • Pro alpha 1(I) colla antibody
      • SEDC antibody
      • Type III collagen antibody
      • VITREORETINOPATHY WITH PHALANGEAL EPIPHYSEAL DYSPLASIA antibody
      see all

    References for Anti-Collagen I + II + III antibody [1.BB.749] (ab61969)

    ab61969 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"