Anti-Collagen I + II + III antibody [NLI/22] (ab53925)

Overview

  • Product nameAnti-Collagen I + II + III antibody [NLI/22]See all Collagen I + II + III primary antibodies ...
  • Description
    Mouse monoclonal [NLI/22] to Collagen I + II + III
  • Specificityab53925 does not react with human collagen types IV, V or VI, human plasma proteins or albumin from various species.
  • Tested applicationsELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Human)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.1% mannitol, 0.1M Sodium chloride, 0.01M Sodium phosphate, pH 7.5
  • Concentration information loading...
  • Purification notesab53925 was purified by Gel Filtration chromatography.
  • Clonality Monoclonal
  • Clone numberNLI/22
  • IsotypeIgM
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab53925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/500 - 1/2000.
ICC/IF 1/5 - 1/20.

Target

  • RelevanceCollagen I is a fibrillar collagen found in most connective tissues, and the only component of the collagen found in cartilage. Mutations in this gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. Type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Collagen type II is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with Collagen I. Mutations in this gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
  • Database links
  • Alternative names
    • AOM antibody
    • COL11A3 antibody
    • COL1A2 antibody
    • COL3A1 antibody
    • Collagen I alpha 1 polypeptide antibody
    • Collagen I alpha 2 polypeptide antibody
    • Collagen II alpha 1 polypeptide antibody
    • Collagen III alpha 1 polypeptide antibody
    • EDS4A antibody
    • OI4 antibody
    see all

References for Anti-Collagen I + II + III antibody [NLI/22] (ab53925)

ab53925 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Purified protein (Collagen type III (also bovine type II collagen))
Specification Collagen type III (also bovine type II collagen)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Type Indirect
Username

Ben Maddox

Verified customer

Submitted Dec 14 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"