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Recombinant fragment: EGSPGRDGSP GAKGDRGETG PAGPPGAPGA PGAPGPVGPA GKSGDRGETG PAGPAGPVGP VGARGPAGPQ GPRGDKGETG EQGDRGIK, corresponding to amino acids 1021 - 1109 of Human Collagen I (NP_000079), with a proprietary tag
Our Abpromise guarantee covers the use of ab88147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 139 kDa.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Sandwich ELISA||Use at an assay dependent concentration.|
IHC-P of ab88147 on human pancreas. antibody concentration 3 ug/ml.
Detection limit for ab88147 is 0.03 ng/ml as a capture antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"